Western Blot (WB)
(ANO1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ANO1 Antibody detected the ANO1 protein (arrow).)
Rabbit anti-Human ANO1 Polyclonal Antibody | anti-ANO1 antibody
ANO1, ID (ANO1, DOG1, ORAOV2, TAOS2, TMEM16A, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2) (Biotin)
Gene Names
ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ANO1; Polyclonal Antibody; ID (ANO1; DOG1; ORAOV2; TAOS2; TMEM16A; Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2) (Biotin); Tumor-amplified and overexpressed sequence 2; anti-ANO1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-ANO1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ANO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-482 amino acids from the Central region of human ANO1.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(ANO1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ANO1 Antibody detected the ANO1 protein (arrow).)
Western Blot (WB)
(ANO1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ANO1 Antibody detected the ANO1 protein (arrow).)
Related Product Information for anti-ANO1 antibody
ANO1 acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity).
Product Categories/Family for anti-ANO1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Barro-Soria, R., et al. Pflugers Arch. 459(3):485-497(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Ferrera, L., et al. J. Biol. Chem. 284(48):33360-33368(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,736 Da
NCBI Official Full Name
anoctamin-1
NCBI Official Synonym Full Names
anoctamin 1
NCBI Official Symbol
ANO1
NCBI Official Synonym Symbols
DOG1; TAOS2; ORAOV2; TMEM16A
NCBI Protein Information
anoctamin-1
UniProt Protein Name
Anoctamin-1
Protein Family
UniProt Gene Name
ANO1
UniProt Synonym Gene Names
DOG1; ORAOV2; TAOS2; TMEM16A
UniProt Entry Name
ANO1_HUMAN
Uniprot Description
ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity
Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport
Research Articles on ANO1
Similar Products
Product Notes
The ANO1 ano1 (Catalog #AAA6273787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANO1, ID (ANO1, DOG1, ORAOV2, TAOS2, TMEM16A, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANO1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ANO1 ano1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
