Western Blot (WB)
(Western Blot: Sample: Human Liver lysate; Primary Ab: 2ug/ml Rabbit Anti-Human ANXA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Annexin A4 (ANXA4) Polyclonal Antibody | anti-ANXA4 antibody
Polyclonal Antibody to Annexin A4 (ANXA4)
MGHHHHHHSGSEF- GNYLDD ALVRQDAQDL YEAGEKKWGT DEVKFLTVLC SRNRNHLLHV FDEYKRISQK DIEQSIKSET SGSFEDALLA IVKCMRNKSA YFAEKLYKSM
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human Liver lysate; Primary Ab: 2ug/ml Rabbit Anti-Human ANXA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Liver lysate; Primary Ab: 2ug/ml Rabbit Anti-Human ANXA4 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(HE staining on IHC-P; Samples: Human Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI Description
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2016]
Uniprot Description
ANXA4: a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Calcium-binding; Lipid-binding
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: cell surface; cytoplasm; extracellular exosome; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; vesicle membrane
Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; identical protein binding; NF-kappaB binding; phospholipase inhibitor activity; protein binding
Biological Process: epithelial cell differentiation; negative regulation of apoptosis; negative regulation of catalytic activity; negative regulation of interleukin-8 secretion; negative regulation of NF-kappaB transcription factor activity; Notch signaling pathway; regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on ANXA4
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Product Notes
The ANXA4 anxa4 (Catalog #AAA2001458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Annexin A4 (ANXA4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A4 (ANXA4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ANXA4 anxa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- GNYLDD ALVRQDAQDL YEAGEKKWGT DEVKFLTVLC SRNRNHLLHV FDEYKRISQK DIEQSIKSET SGSFEDALLA IVKCMRNKSA YFAEKLYKSM. It is sometimes possible for the material contained within the vial of "Annexin A4 (ANXA4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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