Human Annexin A4 (ANXA4) ELISA Kit | ANXA4 elisa kit

Human Annexin A4 (ANXA4) ELISA Kit

Cat. Num. AAA2705613

• Gene Names: ANXA4; ANX4; P32.5; PIG28; PP4-X; ZAP36; PAP-II; HEL-S-274
• Reactivity: Human
• Synonyms: Annexin A4 (ANXA4); Human Annexin A4 (ANXA4) ELISA Kit; ANX-A4; ANX4; PIG28; 35-beta calcimedin; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; Placental anticoagulant protein II; ANXA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A4 (ANXA4).; No significant cross-reactivity or interference between Annexin A4 (ANXA4) and analogues was observed.
• Sequence Length: 321

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A4 (ANXA4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A4 (ANXA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A4 (ANXA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A4 (ANXA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A4 (ANXA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502105
• NCBI GeneID: 307
• NCBI Accession #: NP_001144.1
• NCBI GenBank Nucleotide #: NM_001153.4
• UniProt Accession #: P09525; Q96F33; Q9BWK1; B4DDF9
• Molecular Weight: 27,063 Da
• NCBI Official Full Name: annexin A4 isoform a
• NCBI Official Synonym Full Names: annexin A4
• NCBI Official Symbol: ANXA4
• NCBI Official Synonym Symbols: ANX4; P32.5; PIG28; PP4-X; ZAP36; PAP-II; HEL-S-274
• NCBI Protein Information: annexin A4
• UniProt Protein Name: Annexin A4
• Protein Family: Annexin
• UniProt Gene Name: ANXA4
• UniProt Synonym Gene Names: ANX4; PAP-II

NCBI Description

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2016]

Uniprot Description

ANXA4: a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cell surface; cytoplasm; extracellular exosome; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; vesicle membrane

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; identical protein binding; NF-kappaB binding; phospholipase inhibitor activity; protein binding

Biological Process: epithelial cell differentiation; negative regulation of apoptosis; negative regulation of catalytic activity; negative regulation of interleukin-8 secretion; negative regulation of NF-kappaB transcription factor activity; Notch signaling pathway; regulation of transcription from RNA polymerase II promoter; signal transduction