Rabbit anti-Human, Mouse Bmf Polyclonal Antibody | anti-Bmf antibody

Bmf Antibody

Cat. Num. AAA9411112

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Bmf; Polyclonal Antibody; Bmf Antibody; anti-Bmf antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 184

• Applicable Applications for anti-Bmf antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.
• Target Name: Bmf
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody at 2 ug /ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 ug/mL.)

Related Product Information for anti-Bmf antibody

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Product Categories/Family for anti-Bmf antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15723378
• NCBI GeneID: 90427
• NCBI Accession #: NP_277038
• NCBI GenBank Nucleotide #: NM_033503.3
• Molecular Weight: 14,150 Da
• NCBI Official Full Name: bcl-2-modifying factor isoform bmf-1
• NCBI Official Synonym Full Names: Bcl2 modifying factor
• NCBI Official Symbol: BMF
• NCBI Protein Information: bcl-2-modifying factor
• UniProt Protein Name: Bcl-2-modifying factor
• Protein Family: Bcl-2-modifying factor
• UniProt Gene Name: BMF
• UniProt Entry Name: BMF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

BMF: May play a role in apoptosis. Isoform 1 seems to be the main initiator. Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2. Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. Belongs to the Bcl-2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Apoptosis

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: mitochondrial outer membrane; acrosome; plasma membrane; cytosol; myosin complex

Molecular Function: protein binding

Biological Process: positive regulation of protein homooligomerization; apoptosis; anoikis