Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit | WISP2 elisa kit

Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit

Cat. Num. AAA450278

• Gene Names: WISP2; CCN5; CT58; CTGF-L
• Reactivity: Human
• Synonyms: WNT1 Inducible Signaling Pathway Protein 2 (WISP2); Human WNT1 Inducible Signaling Pathway Protein 2 (WISP2) ELISA Kit; 56 kDa selenium-binding protein; SBP; SBP56; Selenium-binding protein 1; SP56; WISP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WISP2. No significant cross-reactivity or interference between WISP2 and analogues was observed.
• Sequence Length: 218

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.133ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WISP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WISP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WISP2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WISP2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WISP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WISP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WISP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WISP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1020738684
• NCBI GeneID: 8839
• NCBI Accession #: NP_001310298.1
• NCBI GenBank Nucleotide #: NM_001323369.1
• UniProt Accession #: O76076; Q6PEG3; B2R9N4; E1P612
• Molecular Weight: 22,577 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 2 isoform 2
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 2
• NCBI Official Symbol: WISP2
• NCBI Official Synonym Symbols: CCN5; CT58; CTGF-L
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 2
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 2
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP2
• UniProt Synonym Gene Names: CCN5; CT58; CTGFL; WISP-2; CTGF-L

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]

Uniprot Description

May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.