Rabbit anti-Human ANKRD27 Polyclonal Antibody | anti-ANKRD27 antibody
ANKRD27 Conjugated Antibody
Gene Names
ANKRD27; VARP; PP12899
Reactivity
Human
Applications
Most Applications
Synonyms
ANKRD27; Polyclonal Antibody; ANKRD27 Conjugated Antibody; VARP; PP12899; anti-ANKRD27 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ANKRD27 protein.
Form/Format
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
Applicable Applications for anti-ANKRD27 antibody
Most Applications
Application Notes
Suggested Dilution:
AF350 conjugated: most applications: 1:50-1:250
AF405 conjugated: most applications: 1:50-1:250
AF488 conjugated: most applications: 1:50-1:250
AF555 conjugated: most applications: 1:50-1:250
AF594 conjugated: most applications: 1:50-1:250
AF647 conjugated: most applications: 1:50-1:250
AF680 conjugated: most applications: 1:50-1:250
AF750 conjugated: most applications: 1:50-1:250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
AF350 conjugated: most applications: 1:50-1:250
AF405 conjugated: most applications: 1:50-1:250
AF488 conjugated: most applications: 1:50-1:250
AF555 conjugated: most applications: 1:50-1:250
AF594 conjugated: most applications: 1:50-1:250
AF647 conjugated: most applications: 1:50-1:250
AF680 conjugated: most applications: 1:50-1:250
AF750 conjugated: most applications: 1:50-1:250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
Immunogen
Fusion protein of human ANKRD27
Conjugation
Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
Excitation Emission
AF350: 346nm/442nm
Preparation and Storage
Store at 4 degree C for 6 months.
Related Product Information for anti-ANKRD27 antibody
May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
116,984 Da
NCBI Official Full Name
Homo sapiens ankyrin repeat domain 27 (VPS9 domain), mRNA
NCBI Official Synonym Full Names
ankyrin repeat domain 27
NCBI Official Symbol
ANKRD27
NCBI Official Synonym Symbols
VARP; PP12899
NCBI Protein Information
ankyrin repeat domain-containing protein 27
UniProt Protein Name
Ankyrin repeat domain-containing protein 27
Protein Family
UniProt Gene Name
ANKRD27
UniProt Entry Name
ANR27_HUMAN
Uniprot Description
ANKRD27: May be a Rab21 guanine exchange factor and regulate endosome dynamics.
Protein type: GEFs; GEFs, Rab
Chromosomal Location of Human Ortholog: 19q13.11
Cellular Component: cytoplasmic vesicle membrane; membrane; lysosome; cytoplasm; late endosome; early endosome; plasma membrane; melanosome
Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; Rab GTPase binding; GTPase activator activity
Biological Process: protein transport; early endosome to late endosome transport; positive regulation of GTPase activity
Research Articles on ANKRD27
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Product Notes
The ANKRD27 ankrd27 (Catalog #AAA9452134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ANKRD27 Conjugated Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANKRD27 can be used in a range of immunoassay formats including, but not limited to, Most Applications. Suggested Dilution: AF350 conjugated: most applications: 1:50-1:250 AF405 conjugated: most applications: 1:50-1:250 AF488 conjugated: most applications: 1:50-1:250 AF555 conjugated: most applications: 1:50-1:250 AF594 conjugated: most applications: 1:50-1:250 AF647 conjugated: most applications: 1:50-1:250 AF680 conjugated: most applications: 1:50-1:250 AF750 conjugated: most applications: 1:50-1:250 Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000. Researchers should empirically determine the suitability of the ANKRD27 ankrd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANKRD27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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