Immunohistochemistry (IHC)
((10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Goat ANILLIN/Scraps Polyclonal Antibody | anti-ANLN antibody
Goat anti-ANILLIN/Scraps (C Terminus) Antibody
Gene Names
ANLN; scra; FSGS8; Scraps
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ANILLIN/Scraps; Polyclonal Antibody; Goat anti-ANILLIN/Scraps (C Terminus) Antibody; Goat Anti-ANILLIN / Scraps (internal) Antibody; ANLN; Scraps; ANILLIN; anillin; actin binding protein (scraps homolog; Drosophila); anillin (Drosophila Scraps homolog); actin binding protein; DKFZp779A055; scra; ANLN antibody; Scraps antibody; ANILLIN antibody; Drosophila) antibody; actin binding protein antibody; DKFZp779A055 antibody; scra antibody; ANILLIN / Scraps (C Terminus); anti-ANLN antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
WQPDACYKPIGKP
Sequence Length
1124
Applicable Applications for anti-ANLN antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration, 3-10ug/ml.
Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration, 3-10ug/ml.
Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence WQPDACYKPIGKP, from the C Terminus of the protein sequence according to NP_061155.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
((10ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,016 Da
NCBI Official Full Name
actin-binding protein anillin isoform 1
NCBI Official Synonym Full Names
anillin actin binding protein
NCBI Official Symbol
ANLN
NCBI Official Synonym Symbols
scra; FSGS8; Scraps
NCBI Protein Information
actin-binding protein anillin
UniProt Protein Name
Actin-binding protein anillin
Protein Family
UniProt Gene Name
ANLN
UniProt Entry Name
ANLN_HUMAN
NCBI Description
This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
anillin: an actin binding protein that interacts with cleavage furrow proteins such as septins and may play a role in cytokinesis.
Protein type: Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7p15-p14
Cellular Component: actin cytoskeleton; cell-cell adherens junction; contractile ring; intracellular membrane-bound organelle; nucleoplasm
Molecular Function: actin binding
Biological Process: cytokinesis after mitosis; regulation of exit from mitosis; septin ring assembly
Disease: Focal Segmental Glomerulosclerosis 8
Research Articles on ANLN
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Product Notes
The ANLN anln (Catalog #AAA421177) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ANILLIN/Scraps (C Terminus) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ANILLIN/Scraps can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration, 3-10ug/ml. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the ANLN anln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: WQPDACYKPI GKP. It is sometimes possible for the material contained within the vial of "ANILLIN/Scraps, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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