Rabbit anti-Human, Mouse ANGPTL3 Polyclonal Antibody | anti-ANGPTL3 antibody

ANGPTL3, NT (Angiopoietin-related Protein 3, Angiopoietin-like Protein 3, Angiopoietin-5, ANG-5, UNQ153/PRO179, ANGPT5) (FITC)

Cat. Num. AAA6463068

• Gene Names: ANGPTL3; ANL3; ANG-5; FHBL2; ANGPT5
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ANGPTL3; Polyclonal Antibody; NT (Angiopoietin-related Protein 3; Angiopoietin-like Protein 3; Angiopoietin-5; ANG-5; UNQ153/PRO179; ANGPT5) (FITC); anti-ANGPTL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANGPTL3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ANGPTL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ANGPTL3 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ANGPTL3 Antibody (N-term) in CEM cell line lysates (35ug/lane). ANGPTL3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ANGPTL3 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). ANGPTL3 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ANGPTL3 antibody

Antibodies; Angiopoietin

References

1. Shan, L., J. Biol. Chem. 284(3), 1419-1424 (2009). 2. Shimamura, M., Biochem. Biophys. Res. Commun. 301(2), 604-609 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 7656888
• NCBI GeneID: 27329
• NCBI Accession #: NP_055310
• NCBI GenBank Nucleotide #: NM_014495.3
• UniProt Accession #: Q9Y5C1; A0JLS0; B1ALJ0; B2RCW1
• Molecular Weight: Predicted: 51 kDa; ; Observed: 53 kDa
• NCBI Official Full Name: angiopoietin-related protein 3 preproprotein
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: ANGPTL3
• NCBI Official Synonym Symbols: ANL3; ANG-5; FHBL2; ANGPT5
• NCBI Protein Information: angiopoietin-related protein 3; angiopoietin 5
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL3
• UniProt Synonym Gene Names: ANGPT5; ANG-5
• UniProt Entry Name: ANGL3_HUMAN

NCBI Description

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Feb 2013]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Secreted; Cell adhesion; Inhibitor; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: extracellular space; cell surface

Molecular Function: integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity

Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; sequestering of lipid; positive regulation of angiogenesis; cholesterol homeostasis; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis

Disease: Hypobetalipoproteinemia, Familial, 2