Immunoprecipitation (IP)
(Ang2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-Ang2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Ang2 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)
Rabbit anti-Human Angiopoietin-2 Polyclonal Antibody | anti-ANGPT2 antibody
Anti-Angiopoietin-2 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(Ang2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-Ang2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Ang2 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)
Immunoprecipitation (IP)
(Ang2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-Ang2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Ang2 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size: 57 kDa)
Western Blot (WB)
(Anti-Ang2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDaObserved band size:70 kDa)
Western Blot (WB)
(Anti-Ang2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:56 kDaObserved band size:70 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus
Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding
Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; negative regulation of angiogenesis; positive regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; response to activity; angiogenesis; blood coagulation; leukocyte migration
Research Articles on ANGPT2
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Product Notes
The ANGPT2 angpt2 (Catalog #AAA8103541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Angiopoietin-2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the ANGPT2 angpt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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