Mouse Integrin Alpha M (ITGaM) ELISA Kit | ITGaM elisa kit

Mouse Integrin Alpha M (ITGaM) ELISA Kit

Cat. Num. AAA450132

• Gene Names: Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• Reactivity: Mouse
• Synonyms: Integrin Alpha M (ITGaM); Mouse Integrin Alpha M (ITGaM) ELISA Kit; CD11b; ITG-AM; CR3A; MAC-1; MAC1A; MO1A; Complement Component 3 Receptor 3 Subunit; Cell surface glycoprotein MAC-1 subunit alpha; Neutrophil adherence receptor; ITGaM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGaM. No significant cross-reactivity or interference between ITGaM and analogues was observed.
• Sequence Length: 1153

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.05ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGaM were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGaM were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGaM elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITGaM in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITGaM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITGaM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGaM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 124956
• NCBI GeneID: 16409
• UniProt Accession #: P05555; Q8CA73
• Molecular Weight: 115,196 Da
• NCBI Official Full Name: Integrin alpha-M
• NCBI Official Synonym Full Names: integrin alpha M
• NCBI Official Symbol: Itgam
• NCBI Official Synonym Symbols: CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• NCBI Protein Information: integrin alpha-M
• UniProt Protein Name: Integrin alpha-M
• Protein Family: Integrin
• UniProt Gene Name: Itgam

Uniprot Description

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.