Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Dog Angiopoietin 1 (ANGPT1) Polyclonal Antibody | anti-ANGPT1 antibody
Polyclonal Antibody to Angiopoietin 1 (ANGPT1)
Gene Names
ANGPT1; ANG1
Reactivity
Dog
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Angiopoietin 1 (ANGPT1); Polyclonal Antibody; Polyclonal Antibody to Angiopoietin 1 (ANGPT1); anti-ANGPT1 antibody
Host
Rabbit
Reactivity
Dog
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ANGPT1. It has been selected for its ability to recognize ANGPT1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- TKEVL LKGGKKEEEK PFRDCADVYQ AGFNKSGIYT IYINNMPEPK KVFCNMDVNG GGWTVIQHRE DGSLDFQRGW KEYKMGFGNP SGEYWLGNEF IFAITSQRQY TLRIELMDCE GNRAYSQYDR FHIGNEKQNY RLYLKCHTGT AGKQSSLILH GADFSTKDAD NDNCMCKCAL MLTGGWWFDA CGPSNLNGMF YTAGQNHGKL NGIKWHYFKG PSYSLRSTTM MIRPLDF
MGHHHHHHSGSEF- TKEVL LKGGKKEEEK PFRDCADVYQ AGFNKSGIYT IYINNMPEPK KVFCNMDVNG GGWTVIQHRE DGSLDFQRGW KEYKMGFGNP SGEYWLGNEF IFAITSQRQY TLRIELMDCE GNRAYSQYDR FHIGNEKQNY RLYLKCHTGT AGKQSSLILH GADFSTKDAD NDNCMCKCAL MLTGGWWFDA CGPSNLNGMF YTAGQNHGKL NGIKWHYFKG PSYSLRSTTM MIRPLDF
Sequence Length
497
Applicable Applications for anti-ANGPT1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ANGPT1 (Thr266~Phe497) expressed in E.coli.
Cross Reactivity
Dog
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2034872
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,415 Da
NCBI Official Full Name
angiopoietin-1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1
NCBI Official Synonym Symbols
ANG1
NCBI Protein Information
angiopoietin-1
UniProt Protein Name
Angiopoietin-1
Protein Family
UniProt Gene Name
ANGPT1
UniProt Synonym Gene Names
ANG-1
Uniprot Description
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development ().
Similar Products
Product Notes
The ANGPT1 angpt1 (Catalog #AAA2007051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Angiopoietin 1 (ANGPT1) reacts with Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin 1 (ANGPT1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ANGPT1 angpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- TKEVL LKGGKKEEEK PFRDCADVYQ AGFNKSGIYT IYINNMPEPK KVFCNMDVNG GGWTVIQHRE DGSLDFQRGW KEYKMGFGNP SGEYWLGNEF IFAITSQRQY TLRIELMDCE GNRAYSQYDR FHIGNEKQNY RLYLKCHTGT AGKQSSLILH GADFSTKDAD NDNCMCKCAL MLTGGWWFDA CGPSNLNGMF YTAGQNHGKL NGIKWHYFKG PSYSLRSTTM MIRPLDF. It is sometimes possible for the material contained within the vial of "Angiopoietin 1 (ANGPT1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
