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Western Blot (WB) (AMY2A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the AMY2A antibody detected the AMY2A protein (arrow).)

Rabbit anti-Human, Mouse AMY2A Polyclonal Antibody | anti-AMY2A antibody

AMY2A, CT (AMY2A, Pancreatic alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase) (FITC)

Gene Names
AMY2A; PA; AMY2; AMY2B
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AMY2A; Polyclonal Antibody; CT (AMY2A; Pancreatic alpha-amylase; 1; 4-alpha-D-glucan glucanohydrolase) (FITC); 4-alpha-D-glucan glucanohydrolase; anti-AMY2A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-AMY2A antibody
Immunofluorescence (IF), FLISA, Western Blot (WB)
Application Notes
IF: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
AMY2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 491-521 amino acids from the C-terminal region of human AMY2A.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AMY2A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the AMY2A antibody detected the AMY2A protein (arrow).)

Western Blot (WB) (AMY2A Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the AMY2A antibody detected the AMY2A protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of AMY2A Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of AMY2A Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-AMY2A antibody
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.
Product Categories/Family for anti-AMY2A antibody
References
Endo, T., et al. Diabetes 58(3):732-737(2009). Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005). Scheil, H.G., et al. Anthropol Anz 62(4):429-434(2004). Numao, S., et al. J. Biol. Chem. 279(46):48282-48291(2004). Baltova, S., et al. Coll Antropol 28 SUPPL 2, 291-295 (2004): Jacob, M., et al. Biochem. Cell Biol. 70 (10-11), 1105-1114 (1992): Groot, P.C., et al. Genomics 8(1):97-105(1990) Horii, A., et al. Gene 60(1):57-64(1987) Nishide, T., et al. Gene 41 (2-3), 299-304 (1986): Kaczmarek, M.J., et al. Clin. Chim. Acta 79(1):69-73(1977).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
279
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,000
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2; AMY2B
NCBI Protein Information
pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA
UniProt Entry Name
AMYP_HUMAN

NCBI Description

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.2.1.1; Secreted; Hydrolase; Secreted, signal peptide; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space; extracellular region

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

Research Articles on AMY2A

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Product Notes

The AMY2A amy2a (Catalog #AAA6273403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMY2A, CT (AMY2A, Pancreatic alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMY2A can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AMY2A amy2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMY2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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