Western Blot (WB)
(Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody.|Lane 1: TSC22D3 transfected lysate (22.2kD).|Lane 2: Non-transfected lysate.)
Mouse anti-Human TSC22D3 Monoclonal Antibody | anti-TSC22D3 antibody
TSC22D3 (TSC22 domain family protein 3, Glucocorticoid-induced leucine zipper protein, Delta sleep-inducing peptide immunoreactor, DSIP-immunoreactive Peptide, Protein DIP, TSC-22-like Protein, TSC-22-related Protein, TSC-22R, DSIPI, GILZ)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Western Blot (WB)
(Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody.|Lane 1: TSC22D3 transfected lysate (22.2kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody.|Lane 1: TSC22D3 transfected lysate (22.2kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell . [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell . [antibody concentration 10ug/ml].)
Immunoprecipitation (IP)
(Immunoprecipitation of TSC22D3 transfected lysate using TSC22D3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TSC22D3 rabbit polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of TSC22D3 transfected lysate using TSC22D3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TSC22D3 rabbit polyclonal antibody.)
Testing Data
(Detection limit for recombinant GST tagged TSC22D3 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged TSC22D3 is ~0.03ng/ml as a capture antibody.)
Western Blot (WB)
(Western Blot detection against Immunogen (36.41kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.41kD).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities
By similarity. Ref.15
Subunit structure: Can form homodimers, however it is likely to function as a monomer. Interacts with AP1
By similarity. Interacts with NFKB1. Ref.13 Ref.14
Tissue specificity: Expressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells. Ref.2 Ref.14
Induction: By glucocorticoids in lymphoid cells and upon IL4, IL10, IL13 or glucocorticoid treatment in monocyte/macrophage cells. Transiently induced by IL2 deprivation in T-cells. Ref.14 Ref.15
Domain: The leucine-zipper is involved in homodimerization
By similarity.
Sequence similarities: Belongs to the TSC-22/Dip/Bun family.
Sequence caution: The sequence AAH18148.3 differs from that shown. Reason: Erroneous initiation. The sequence CAA90644.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB53669.1 differs from that shown. Reason: Frameshift at position 4.
Research Articles on TSC22D3
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Product Notes
The TSC22D3 tsc22d3 (Catalog #AAA646546) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TSC22D3 (TSC22 domain family protein 3, Glucocorticoid-induced leucine zipper protein, Delta sleep-inducing peptide immunoreactor, DSIP-immunoreactive Peptide, Protein DIP, TSC-22-like Protein, TSC-22-related Protein, TSC-22R, DSIPI, GILZ) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TSC22D3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the TSC22D3 tsc22d3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAQSKLDCRS PVGLDCCNCC LDLAHRSGLQ RGSSGENNNP GSPTVSNFRQ LQEKLVFENL NTDKLNSIMR QDSLEPVLRD PCYLINEGIC NRNIDQT. It is sometimes possible for the material contained within the vial of "TSC22D3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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