Western Blot (WB)
(Western Blot;Sample: Recombinant Amphiphysin, Human.)
Rabbit anti-Human Amphiphysin Polyclonal Antibody | anti-AMPH antibody
Rabbit anti-Human Amphiphysin Polyclonal Antibody
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot;Sample: Recombinant Amphiphysin, Human.)
Western Blot (WB)
(Western Blot;Sample: Recombinant Amphiphysin, Human.)
Western Blot (WB)
(Western Blot;Sample: Human U2OS cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Human AMPH AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot;Sample: Human U2OS cell lysate;Primary Ab: 1ug/ml Rabbit Anti-Human AMPH AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]
Uniprot Description
amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 7p14.1
Cellular Component: actin cytoskeleton; cell junction; cytosol; synaptic vesicle; synaptic vesicle membrane
Molecular Function: phospholipid binding; protein binding
Biological Process: endocytosis; synaptic transmission
Research Articles on AMPH
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Product Notes
The AMPH amph (Catalog #AAA2032882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human Amphiphysin Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Amphiphysin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the AMPH amph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amphiphysin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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