Mouse anti-Rat AMACR Monoclonal Antibody | anti-AMACR antibody

AMACR (C-terminus) Monoclonal Antibody

Cat. Num. AAA3014276

• Gene Names: AMACR; RM; RACE; CBAS4; P504S; AMACRD
• Reactivity: Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AMACR; Monoclonal Antibody; AMACR (C-terminus) Monoclonal Antibody; Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; anti-AMACR antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Specificity: This antibody detects endogenous levels of AMACR and does not cross-react with related proteins
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 394

• Applicable Applications for anti-AMACR antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:50-200
• Immunogen: Synthetic peptide, corresponding to the C-terminus of Human AMACR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot detection of AMACR (C-terminus) antibody in Rat Liver lysates using AMACR (C-terminus) antibody (1:1000 diluted).)

Testing Data

Related Product Information for anti-AMACR antibody

P504S, also known as AMACR (a-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal b-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy.

NCBI and Uniprot Product Information

• NCBI GI #: 266456254
• NCBI GeneID: 23600
• NCBI Accession #: NP_001161067.1
• NCBI GenBank Nucleotide #: NP_001161067.1
• UniProt Accession #: Q9UHK6
• Molecular Weight: Predicted Band Size: 42KDa ; Observed Band Size: 42KDa
• NCBI Official Full Name: alpha-methylacyl-CoA racemase isoform 3
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: AMACR
• NCBI Official Synonym Symbols: RM; RACE; CBAS4; P504S; AMACRD
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: AMACR
• UniProt Entry Name: AMACR_HUMAN

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]