Rabbit anti-Human ALX4 Polyclonal Antibody | anti-ALX4 antibody

ALX4, ID (ALX4, KIAA1788, Homeobox protein aristaless-like 4) (FITC)

Cat. Num. AAA6273117

• Gene Names: ALX4; CRS5; FND2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALX4; Polyclonal Antibody; ID (ALX4; KIAA1788; Homeobox protein aristaless-like 4) (FITC); Homeobox protein aristaless-like 4; anti-ALX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ALX4 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ALX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-283 amino acids from the Central region of human ALX4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ALX4 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ALX4 antibody detected the ALX4 protein (arrow).)

Flow Cytometry (FC/FACS)

(ALX4 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ALX4 antibody

This gene encodes a paired-like homeodomain transcription factor expressed in the mesenchyme of developing bones, limbs, hair, teeth, and mammary tissue. Mutations in this gene cause parietal foramina 2 (PFM2); an autosomal dominant disease characterized by deficient ossification of the parietal bones. Mutations in this gene also cause a form of frontonasal dysplasia with alopecia and hypogonadism; suggesting a role for this gene in craniofacial development, mesenchymal-epithelial communication, and hair follicle development. Deletion of a segment of chromosome 11 containing this gene, del(11)(p11p12), causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies, mental retardation, multiple exostoses, and genital abnormalities in males. In mouse, this gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq].

Product Categories/Family for anti-ALX4 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Tanzer, M., et al. PLoS ONE 5 (2), E9061 (2010): Kayserili, H., et al. Hum. Mol. Genet. 18(22):4357-4366(2009). Chang, H., et al. J. Clin. Pathol. 62(10):908-914(2009). Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 55743092
• NCBI GeneID: 60529
• NCBI Accession #: NP_068745.2
• NCBI GenBank Nucleotide #: NM_021926.3
• UniProt Accession #: Q9H161; Q96JN7; Q9H198; Q9HAY9
• Molecular Weight: 44,241 Da
• NCBI Official Full Name: homeobox protein aristaless-like 4
• NCBI Official Synonym Full Names: ALX homeobox 4
• NCBI Official Symbol: ALX4
• NCBI Official Synonym Symbols: CRS5; FND2
• NCBI Protein Information: homeobox protein aristaless-like 4
• UniProt Protein Name: Homeobox protein aristaless-like 4
• Protein Family: Homeobox protein
• UniProt Gene Name: ALX4
• UniProt Synonym Gene Names: KIAA1788
• UniProt Entry Name: ALX4_HUMAN

NCBI Description

This gene encodes a paired-like homeodomain transcription factor expressed in the mesenchyme of developing bones, limbs, hair, teeth, and mammary tissue. Mutations in this gene cause parietal foramina 2 (PFM2); an autosomal dominant disease characterized by deficient ossification of the parietal bones. Mutations in this gene also cause a form of frontonasal dysplasia with alopecia and hypogonadism; suggesting a role for this gene in craniofacial development, mesenchymal-epithelial communication, and hair follicle development. Deletion of a segment of chromosome 11 containing this gene, del(11)(p11p12), causes Potocki-Shaffer syndrome (PSS); a syndrome characterized by craniofacial anomalies, cognitive disability, multiple exostoses, and genital abnormalities in males. In mouse, this gene has been shown to use dual translation initiation sites located 16 codons apart. [provided by RefSeq, Oct 2009]