Mouse alpha-tubulin Monoclonal Antibody | anti-TUBA1A antibody

alpha-tubulin Mouse mAb

Cat. Num. AAA9602855

• Gene Names: TUBA1A; LIS3; TUBA3; B-ALPHA-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, ELISA
• Purity: Affinity Purification
• Synonyms: alpha-tubulin; Monoclonal Antibody; alpha-tubulin Mouse mAb; TUBA1A; Alpha-tubulin 3; B-ALPHA-1; Hum-a-tub1; TUBA3; Tubulin alpha-3 chain; Tubulin B-alpha-1; Tubulin; alpha 1a; alpha; brain-specific; Hum-a-tub2; LIS3; Tubulin Alpha 1a; Tubulin alpha-1A chain; anti-TUBA1A antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Specificity: The alpha-tubulin mouse monoclonal antibody can detect endogenous alpha-tubulin proteins.
• Purity/Purification: Affinity Purification
• Form/Format: 1mg/ml in PBS, pH 7.4.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBA1A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; IP: 1:200; IF: 1:200
• Immunogen: Mouse monoclonal antibody is prepared by immunizing recombinant protein.
• Subcellular Location: Cytoplasm, Cytoskeleton.
• Tissue Specificity: Expressed at a high level in fetal brain.

Western Blot (WB)

(Western blot analysis of Mouse Brain Tissue with alpha-tubulin Mouse mAb diluted at 1:5, 000.)

Related Product Information for anti-TUBA1A antibody

Description: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of alpha-and beta-tubulin.
Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit Structure: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with SETD2; the interaction is independent on alpha-tubulin acetylation on Lys-40.
Post-translational Modifications: Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly. Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. Nitration of Tyr-451 is irreversible and interferes with normal dynein intracellular distribution. Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Tubulin alpha-1A chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1. Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003, PubMed:26968983). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (PubMed:26968983).Detyrosinated tubulin alpha-1A chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity).
Similarity: Belongs to the tubulin family.

NCBI and Uniprot Product Information

• NCBI GI #: 393715091
• NCBI GeneID: 7846
• NCBI Accession #: NP_001257328.1
• NCBI GenBank Nucleotide #: NM_001270399.1
• UniProt Accession #: Q71U36; P04687; P05209; A8K0B8; G3V1U9
• Molecular Weight: Observed: 52 kD; Predicted: 51 kDa
• NCBI Official Full Name: tubulin alpha-1A chain isoform 1
• NCBI Official Synonym Full Names: tubulin alpha 1a
• NCBI Official Symbol: TUBA1A
• NCBI Official Synonym Symbols: LIS3; TUBA3; B-ALPHA-1
• NCBI Protein Information: tubulin alpha-1A chain
• UniProt Protein Name: Tubulin alpha-1A chain
• Protein Family: Alpha-tubulin N-acetyltransferase
• UniProt Gene Name: TUBA1A
• UniProt Synonym Gene Names: TUBA3

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.