Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Alpha-Fetoprotein Polyclonal Antibody | anti-aFP antibody
Polyclonal Antibody to Alpha-Fetoprotein (aFP)
Gene Names
Tpsab1; Mcpt7; TPSB1; rMCP-7
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Alpha-Fetoprotein; Polyclonal Antibody; Polyclonal Antibody to Alpha-Fetoprotein (aFP); anti-aFP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against aFP. It has beenselected for its ability to recognize aFP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-aFP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
aFP (Ile31~Ser576)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant aFP (Ile31~Ser576) expressed in E Coli.
Conjugated Antibody
The Cy3 conjugated antibody version of this item is also available as catalog #MBS2038909
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: aFP knockout HepG2 cell lysate;;Predicted MW: 69kDa ;Observed MW: 69kDa;Primary Ab: 2ug/ml Rabbit Anti-Human aFP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: aFP knockout HepG2 cell lysate;;Predicted MW: 69kDa ;Observed MW: 69kDa;Primary Ab: 2ug/ml Rabbit Anti-Human aFP Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))
Product Categories/Family for anti-aFP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,400 Da
NCBI Official Full Name
tryptase
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
Tpsab1
NCBI Official Synonym Symbols
Mcpt7; TPSB1; rMCP-7
NCBI Protein Information
tryptase
UniProt Protein Name
Tryptase
Protein Family
UniProt Gene Name
Tpsab1
UniProt Synonym Gene Names
Mcp7; Mcpt7; Tpsb1; rMCP-7
NCBI Description
serine protease in mast cells [RGD, Feb 2006]
Uniprot Description
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity ().
Research Articles on aFP
Similar Products
Product Notes
The aFP tpsab1 (Catalog #AAA2028655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alpha-Fetoprotein (aFP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-Fetoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the aFP tpsab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Fetoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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