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Western Blot (WB) (Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse aHSG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Alpha-2-Heremans Schmid Glycoprotein (aHSG) Polyclonal Antibody | anti-aHSG antibody

Polyclonal Antibody to Alpha-2-Heremans Schmid Glycoprotein (aHSG)

Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Alpha-2-Heremans Schmid Glycoprotein (aHSG); Polyclonal Antibody; Polyclonal Antibody to Alpha-2-Heremans Schmid Glycoprotein (aHSG); anti-aHSG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against aHSG. It has been selected for its ability to recognize aHSG in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-AP QGTGLGFREL ACDDPEAEQV ALLAVDYLNN HLLQGFKQVL NQIDKVKVWS RRPFGVVYEM EVDTLETTCH ALDPTPLANC SVRQLTEHAV EGDCDFHILK QDGQFRVMHT QCHSTPDSAE DVRKLCPRCP LLTPFNDTNV VHTVNTALAA FNTQNNGTYF KLVEISRAQN VPLPVSTLVE FVIAATDCTA KEVTDPAKCN LLAEKQHGFC KANLMHNLGG EEVSVACKLF
Applicable Applications for anti-aHSG antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant aHSG (Ala19~Phe250) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2039207
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse aHSG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse aHSG Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Ovary Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Ovary Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,326 Da
NCBI Official Full Name
alpha-2-HS-glycoprotein isoform 1
NCBI Official Synonym Full Names
alpha-2-HS-glycoprotein
NCBI Official Symbol
Ahsg
NCBI Protein Information
alpha-2-HS-glycoprotein
UniProt Protein Name
Alpha-2-HS-glycoprotein
Protein Family
UniProt Gene Name
Ahsg
UniProt Synonym Gene Names
Fetua

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16 B1|16 13.74 cM

Cellular Component: blood microparticle; extracellular exosome; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; protein complex

Molecular Function: cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; receptor signaling protein tyrosine kinase inhibitor activity

Biological Process: acute-phase response; male gonad development; negative regulation of bone mineralization; negative regulation of cell growth; negative regulation of insulin receptor signaling pathway; ossification; positive regulation of bone resorption; positive regulation of phagocytosis; protein complex assembly; regulation of inflammatory response

Research Articles on aHSG

Similar Products

Product Notes

The aHSG ahsg (Catalog #AAA2003632) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alpha-2-Heremans Schmid Glycoprotein (aHSG) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-2-Heremans Schmid Glycoprotein (aHSG) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the aHSG ahsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-AP QGTGLGFREL ACDDPEAEQV ALLAVDYLNN HLLQGFKQVL NQIDKVKVWS RRPFGVVYEM EVDTLETTCH ALDPTPLANC SVRQLTEHAV EGDCDFHILK QDGQFRVMHT QCHSTPDSAE DVRKLCPRCP LLTPFNDTNV VHTVNTALAA FNTQNNGTYF KLVEISRAQN VPLPVSTLVE FVIAATDCTA KEVTDPAKCN LLAEKQHGFC KANLMHNLGG EEVSVACKLF. It is sometimes possible for the material contained within the vial of "Alpha-2-Heremans Schmid Glycoprotein (aHSG), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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