Western Blot (WB)
(Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase alpha1 (S23) Polyclonal Antibody)
Rabbit anti-Rat alpha 1 Sodium Potassium ATPase Polyclonal Antibody | anti-atp1a1 antibody
phospho-alpha 1 Sodium Potassium ATPase (Ser23) Rabbit pAb
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
Purity
Affinity Chromatography
Synonyms
alpha 1 Sodium Potassium ATPase; Polyclonal Antibody; phospho-alpha 1 Sodium Potassium ATPase (Ser23) Rabbit pAb; ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; anti-atp1a1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Na+/K+-ATPase alpha1 (S23) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase alpha1 protein only when phosphorylated at S23.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-atp1a1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:100-1:300
ELISA: 1:5000
IF: 1:100-1:300
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from rat ATP1 alpha1/Na+K+ ATPase1 around the phosphorylation site of Ser23. AA range:15-64
Conjugation
Unconjugated
Modification
Phosphoralated
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase alpha1 (S23) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of rat cells using Phospho-Na+/K+-ATPase alpha1 (S23) Polyclonal Antibody)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from rat brain, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from rat brain, using ATP1 alpha1/Na+K+ ATPase1 (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.)
Related Product Information for anti-atp1a1 antibody
The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-atp1a1 antibody
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Product Notes
The atp1a1 (Catalog #AAA4755347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The phospho-alpha 1 Sodium Potassium ATPase (Ser23) Rabbit pAb reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha 1 Sodium Potassium ATPase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:300 ELISA: 1:5000. Researchers should empirically determine the suitability of the atp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha 1 Sodium Potassium ATPase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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