IGJ / Immunoglobulin J chain Recombinant Protein | IGJ recombinant protein

Recombinant Human IGJ / Immunoglobulin J chain Protein (His tag)

Cat. Num. AAA2545432

• Gene Names: JCHAIN; IGJ; JCH; IGCJ
• Purity: > 90 % as determined by reducing SDS-PAGE.
• Synonyms: IGJ / Immunoglobulin J chain; Recombinant Human IGJ / Immunoglobulin J chain Protein (His tag); IGCJ; JCH; IGJ recombinant protein

• Host: E. coli
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

• Species: Human
• Sequence: Gln 23-Asp 159
• Tag: C-His
• Endotoxin: Please contact us for more information.
• Reconstitution: It is recommended that sterile water(400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for IGJ recombinant protein

Immunoglobulin J chain, also known as IGJ and IGCJ, is a secreted polypeptide which is the first immunoglobulin-related polypeptide expressed during the embryogenesis and differentiation of B cells in the fetal liver. The joining Immunoglobulin J chain is a small polypeptide, expressed by mucosal and glandular plasma cells, which regulates polymer formation of immunoglobulin (Ig)A and IgM. Immunoglobulin J chain / IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. Immunoglobulin J chain / IGJ also help to bind these immunoglobulins to secretory component. J-chain incorporation into polymeric IgA (pIgA, mainly dimers) and pentameric IgM endows these antibodies with several salient features. Immunoglobulin J chain / IGJ is involved in creating the binding site for pIgR / SC in the Ig polymers, not only by determining the polymeric quaternary structure but apparently also by interacting directly with the receptor protein. Both the immunoglobulin J chain / IGJ and the pIgR/SC are key proteins in secretory immunity.

NCBI and Uniprot Product Information

• NCBI GI #: 21489959
• NCBI GeneID: 3512
• NCBI Accession #: NP_653247.1
• NCBI GenBank Nucleotide #: NM_144646.3
• UniProt Accession #: P01591
• Molecular Weight: Mol_Mass: 17 kDa; AP_Mol_Mass: 26 kDa
• NCBI Official Full Name: immunoglobulin J chain
• NCBI Official Synonym Full Names: joining chain of multimeric IgA and IgM
• NCBI Official Symbol: JCHAIN
• NCBI Official Synonym Symbols: IGJ; JCH; IGCJ
• NCBI Protein Information: immunoglobulin J chain
• UniProt Protein Name: Immunoglobulin J chain
• UniProt Gene Name: JCHAIN

Uniprot Description

IGJ: Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular region; extracellular space

Molecular Function: IgA binding; peptidoglycan binding; phosphatidylcholine binding; protein homodimerization activity; single-stranded DNA binding

Biological Process: adaptive immune response; antibacterial humoral response; glomerular filtration; innate immune response; positive regulation of protein oligomerization; receptor-mediated endocytosis; retinal homeostasis