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Western Blot (WB) (Western Blot: Sample: Human Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALPPL2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) Polyclonal Antibody | anti-ALPPL2 antibody

Polyclonal Antibody to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2)

Gene Names
ALPPL2; ALPG; GCAP; ALPPL
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Alkaline Phosphatase; Placental Like Protein 2 (ALPPL2); Polyclonal Antibody; Polyclonal Antibody to Alkaline Phosphatase; anti-ALPPL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ALPPL2. It has been selected for its ability to recognize ALPPL2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGR
Sequence Length
532
Applicable Applications for anti-ALPPL2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ALPPL2 (Ile20~Arg333) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2081560
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALPPL2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Human Placenta lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALPPL2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
251
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,377 Da
NCBI Official Full Name
alkaline phosphatase, placental-like preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental like 2
NCBI Official Symbol
ALPPL2
NCBI Official Synonym Symbols
ALPG; GCAP; ALPPL
NCBI Protein Information
alkaline phosphatase, placental-like
UniProt Protein Name
Alkaline phosphatase, placental-like
Protein Family
UniProt Gene Name
ALPPL2
UniProt Synonym Gene Names
ALPPL; GCAP; PLAP-like

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPPL2: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008]

Protein type: Cofactor and Vitamin Metabolism - folate biosynthesis; EC 3.1.3.1; Membrane protein, GPI anchor; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: extracellular region; plasma membrane

Molecular Function: alkaline phosphatase activity; metal ion binding

Biological Process: C-terminal protein lipidation; dephosphorylation

Research Articles on ALPPL2

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Product Notes

The ALPPL2 alppl2 (Catalog #AAA2002888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALPPL2 alppl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG SEF-I IPVEEENPDF WNRQAAEALG AAKKLQPAQT AAKNLIIFLG DGMGVSTVTA ARILKGQKKD KLGPETFLAM DRFPYVALSK TYSVDKHVPD SGATATAYLC GVKGNFQTIG LSAAARFNQC NTTRGNEVIS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADVPASARQE GCQDIATQLI SNMDIDVILG GGRKYMFPMG TPDPEYPDDY SQGGTRLDGK NLVQEWLAKH QGARYVWNRT ELLQASLDPS VTHLMGLFEP GDMKYEIHRD STLDPSLMEM TEAALLLLSR NPRGFFLFVE GGR. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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