anti-Human BMP4 Antibody | anti-BMP4 antibody

Goat anti-BMP4 Antibody

Cat. Num. AAA423787

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: BMP4; Goat anti-BMP4 Antibody; bone morphogenetic protein 4; BMP2B; BMP2B1; MCOPS6; OFC11; ZYME; BMP-2B; BMP-4; bone morphogenetic protein 2B; anti-BMP4 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 408

• Applicable Applications for anti-BMP4 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:64000.; WB: Approx 40-45kDa band observed in lysates of cell lines A431 and HeLa. Recommended concentration: 0.3-1ug/ml.
• Immunogen Sequence: Peptide with sequence C-SPKHHSQRARK, from the internal region of the protein sequence according to NP_001193.2.
• Peptide Sequence + C: C-SPKHHSQRARK
• Epitope: Internal Region
• Note: The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_570911.2, NP_001193.2, NP_570912.2.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((0.3ug/ml) staining of A431 (A) and HeLa (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193.2
• NCBI GenBank Nucleotide #: NM_001202.5
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 46.6kDa
• NCBI Official Full Name: bone morphogenetic protein 4 isoform a preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction ().