anti-Human ALK Polyclonal Antibody | anti-ALK antibody

Anti-ALK (Phospho-Tyr1586) Rabbit pAb

Cat. Num. AAA477144

• Gene Names: ALK; CD246; NBLST3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: ALK; Polyclonal Antibody; Anti-ALK (Phospho-Tyr1586) Rabbit pAb; Alk; ALK tyrosine kinase receptor; ALK Tyrosine Kinase Receptor Precursor; ALK/EML4 fusion gene; included; ALK/NPM1 fusion gene; ALK_HUMAN; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; anaplastic lymphoma receptor tyrosine kinase; CD 246; CD246; CD246 Antigen; EC 2.7.10.1; Ki 1; Ki1; mutant anaplastic lymphoma kinase; NBLST 3; NBLST3; Tcrz; TFG/ALK; anti-ALK antibody

• Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: 10mM PBS with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 1620

• Applicable Applications for anti-ALK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ALK around the phosphorylation site of tyrosine 1586 (Y-G-YP-Q-Q).
• Sensitivity: This antibody detects endogenous levels of total ALK protein.
• Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 1 LDL-receptor class A domain. Contains 2 MAM domains. Contains 1 protein kinase domain.
• Subunit Structure: Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1.
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using ALK (Phospho-Tyr1586) Antibody.)

Related Product Information for anti-ALK antibody

Entrez Summary: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

NCBI and Uniprot Product Information

• NCBI GI #: 29029632
• NCBI GeneID: 238
• NCBI Accession #: NP_004295.2
• NCBI GenBank Nucleotide #: NM_004304.4
• UniProt Accession #: Q9UM73; Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6
• Molecular Weight: 176
• NCBI Official Full Name: ALK tyrosine kinase receptor
• NCBI Official Synonym Full Names: anaplastic lymphoma receptor tyrosine kinase
• NCBI Official Symbol: ALK
• NCBI Official Synonym Symbols: CD246; NBLST3
• NCBI Protein Information: ALK tyrosine kinase receptor
• UniProt Protein Name: ALK tyrosine kinase receptor
• Protein Family: Alkylsulfatase
• UniProt Gene Name: ALK

NCBI Description

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Uniprot Description

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: Alk family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 2p23.2-p23.1

Cellular Component: integral to plasma membrane; protein complex

Molecular Function: identical protein binding; NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cell proliferation; neuron development; phosphorylation; protein amino acid autophosphorylation; regulation of apoptosis; signal transduction

Disease: Neuroblastoma, Susceptibility To, 3