Rabbit anti-Human ALK Polyclonal Antibody | anti-ALK antibody

ALK, ID (ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246) (APC)

Cat. Num. AAA6272961

• Gene Names: ALK; CD246; NBLST3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ALK; Polyclonal Antibody; ID (ALK; ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD246) (APC); CD246; anti-ALK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALK
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ALK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644~673 amino acids from the center region of human ALK.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALK(Center)(arrow) using rabbit polyclonal ALK(Center) Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK (Center) gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-ALK antibody

ALK, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t(2;5)(p23;q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.

Product Categories/Family for anti-ALK antibody

Antibodies; Protein Kinases

References

Morris, S.W., et al., Oncogene 14(18):2175-2188 (1997). Iwahara, T., et al., Oncogene 14(4):439-449 (1997). Morris, S.W., et al., Science 263(5151):1281-1284 (1994). Morris, S.W., et al., Oncogene 15, 2883-2883 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 29029632
• NCBI GeneID: 238
• NCBI Accession #: NP_004295.2
• NCBI GenBank Nucleotide #: NM_004304.4
• UniProt Accession #: Q9UM73; Q4ZFX9; Q53QQ6; Q53RZ4; Q59FI3; Q9Y4K6
• Molecular Weight: 176,442 Da
• NCBI Official Full Name: ALK tyrosine kinase receptor
• NCBI Official Synonym Full Names: anaplastic lymphoma receptor tyrosine kinase
• NCBI Official Symbol: ALK
• NCBI Official Synonym Symbols: CD246; NBLST3
• NCBI Protein Information: ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase
• UniProt Protein Name: ALK tyrosine kinase receptor
• Protein Family: Alkylsulfatase
• UniProt Gene Name: ALK
• UniProt Entry Name: ALK_HUMAN

NCBI Description

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Uniprot Description

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; Oncoprotein; EC 2.7.10.1; Protein kinase, TK; TK group; Alk family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: protein complex; integral to plasma membrane

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor

Disease: Neuroblastoma, Susceptibility To, 3