Rabbit anti-Human ALG5 Polyclonal Antibody | anti-ALG5 antibody

ALG5, ID (ALG5, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog) (Azide free) (HRP)

Cat. Num. AAA6272931

• Gene Names: ALG5; bA421P11.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALG5; Polyclonal Antibody; ID (ALG5; Dolichyl-phosphate beta-glucosyltransferase; Asparagine-linked glycosylation protein 5 homolog) (Azide free) (HRP); Asparagine-linked glycosylation protein 5 homolog; anti-ALG5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 324

• Applicable Applications for anti-ALG5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ALG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-100 amino acids from the Central region of human ALG5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ALG5 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ALG5 antibody detected the ALG5 protein (arrow).)

Related Product Information for anti-ALG5 antibody

This gene encodes a member of the glycosyltransferase 2 family. The encoded protein participates in glucosylation of the oligomannose core in N-linked glycosylation of proteins. The addition of glucose residues to the oligomannose core is necessary to ensure substrate recognition, and therefore, effectual transfer of the oligomannose core to the nascent glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-ALG5 antibody

Antibodies; Enzymes

References

Imbach, T., et al. Proc. Natl. Acad. Sci. U.S.A. 96(12):6982-6987(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7019323
• NCBI GeneID: 29880
• NCBI Accession #: NP_037470.1
• NCBI GenBank Nucleotide #: NP_037470.1
• UniProt Accession #: Q9Y673
• NCBI Official Full Name: dolichyl-phosphate beta-glucosyltransferase isoform 1
• NCBI Official Synonym Full Names: ALG5 dolichyl-phosphate beta-glucosyltransferase
• NCBI Official Symbol: ALG5
• NCBI Official Synonym Symbols: bA421P11.2
• NCBI Protein Information: dolichyl-phosphate beta-glucosyltransferase
• UniProt Protein Name: Dolichyl-phosphate beta-glucosyltransferase
• Protein Family: Dolichyl-phosphate beta-glucosyltransferase
• UniProt Gene Name: ALG5
• UniProt Synonym Gene Names: DolP-glucosyltransferase

NCBI Description

This gene encodes a member of the glycosyltransferase 2 family. The encoded protein participates in glucosylation of the oligomannose core in N-linked glycosylation of proteins. The addition of glucose residues to the oligomannose core is necessary to ensure substrate recognition, and therefore, effectual transfer of the oligomannose core to the nascent glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]