Rabbit Aldolase Polyclonal Antibody | anti-ALDOA antibody

Aldolase Polyclonal Antibody

Cat. Num. AAA3012215

• Gene Names: ALDOA; ALDA; GSD12; HEL-S-87p
• Reactivity: Human, Mouse, Rat, Zebrafish
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: Aldolase; Polyclonal Antibody; Aldolase Polyclonal Antibody; ALDOA; ALDA; Aldo1; Aldolase 1; Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; fructose-bisphosphate A; Epididymis secretory sperm binding protein Li 87p; FRUCTOALDOLASE A; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase A; Fructose bisphosphate aldolase; FRUCTOSE-1; 6-BISPHOSPHATE ALDOLASE A; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase A Muscle-type; GSD12; HEL S 87p; Lung cancer antigen NY LU 1; Lung cancer antigen NY-LU-1; MGC10942; MGC17716; MGC17767; Muscle type aldolase; Muscle-type aldolase; RNALDOG5; anti-ALDOA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Aldolase polyclonal antibody detects endogenous levels of Aldolase protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-ALDOA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IF: 1:50-1:100; IHC: 1:50-1:200
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution. Positive control:Lane 1: A549 Lane 2: Hela Lane 3: Mouse liver Lane 4: Rat liverLane 5: Rat spleen)

Immunocytochemistry (ICC)

(ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-ALDOA antibody

Fructose 1, 6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1, 6-bisphosphate. Fructose 1, 6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A; the liver-specific Aldolase B; and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldo-lase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4557305
• NCBI GeneID: 226
• UniProt Accession #: P04075; P05064; P05065
• Molecular Weight: 39kDa
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate A
• NCBI Official Symbol: ALDOA
• NCBI Official Synonym Symbols: ALDA; GSD12; HEL-S-87p
• NCBI Protein Information: fructose-bisphosphate aldolase A
• UniProt Protein Name: Fructose-bisphosphate aldolase A
• Protein Family: Aldolase
• UniProt Gene Name: ALDOA
• UniProt Synonym Gene Names: ALDA
• UniProt Entry Name: ALDOA_HUMAN

NCBI Description

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

Uniprot Description

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Protein type: Carbohydrate Metabolism - fructose and mannose; Lyase; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton

Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding

Biological Process: platelet activation; glycolysis; striated muscle contraction; glucose metabolic process; actin filament organization; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process

Disease: Glycogen Storage Disease Xii