Rabbit anti-Human Aldolase Polyclonal Antibody | anti-ALDOA antibody

Aldolase, CT (Fructose-bisphosphate Aldolase A, Muscle-type Aldolase, Lung Cancer Antigen NY-LU-1, ALDOA, ALDA) (MaxLight 550)

Cat. Num. AAA6462464

• Gene Names: ALDOA; ALDA; GSD12; HEL-S-87p
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Aldolase; Polyclonal Antibody; CT (Fructose-bisphosphate Aldolase A; Muscle-type Aldolase; Lung Cancer Antigen NY-LU-1; ALDOA; ALDA) (MaxLight 550); anti-ALDOA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDOA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-ALDOA antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ALDOA (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ALDOA Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ALDOA(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ALDOA antibody

Antibodies; Enzymes

References

1. Gizak, A., Proteins 72(1), 209-216 (2008). 2. Lu, J., Biochem. Biophys. Res. Commun. 369(3), 948-952 (2008). 3. Valis, K., Mol. Cell. Biochem. 311(1-2), 225-231 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4557305
• NCBI GeneID: 226
• NCBI Accession #: NP_000025
• NCBI GenBank Nucleotide #: NM_000034
• UniProt Accession #: P04075
• Molecular Weight: 39kDa
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate A
• NCBI Official Symbol: ALDOA
• NCBI Official Synonym Symbols: ALDA; GSD12; HEL-S-87p
• NCBI Protein Information: fructose-bisphosphate aldolase A
• UniProt Protein Name: Fructose-bisphosphate aldolase A
• Protein Family: Aldolase
• UniProt Gene Name: ALDOA
• UniProt Synonym Gene Names: ALDA
• UniProt Entry Name: ALDOA_HUMAN

NCBI Description

This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]

Uniprot Description

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Protein type: Carbohydrate Metabolism - fructose and mannose; Lyase; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton

Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding

Biological Process: platelet activation; glycolysis; striated muscle contraction; glucose metabolic process; actin filament organization; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process

Disease: Glycogen Storage Disease Xii