Mouse UBTF/UBF Monoclonal Antibody | anti-UBTF/UBF antibody

Anti-UBTF/UBF Antibody (clone 1A2)

Cat. Num. AAA2400621

• Gene Names: UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• Reactivity: Human; Predicted: Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: UBTF/UBF; Monoclonal Antibody; Anti-UBTF/UBF Antibody (clone 1A2); Mouse Monoclonal [clone 6B6] (IgG2a; k) to Human UBTF/UBF; UBTF; Autoantigen NOR-90; UBF; NOR-90; UBF-1; UBF1; Upstream-binding factor 1; anti-UBTF/UBF antibody

• Host: Mouse
• Reactivity: Human; Predicted: Rat
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 1A2
• Specificity: Human UBTF
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Sequence Length: 764

• Applicable Applications for anti-UBTF/UBF antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 10 ug/ml; IHC-P: 5 ug/ml; WB: 1:500-1:1000
• Immunogen: UBTF (NP_055048, 551 a.a. ~ 651 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-UBTF/UBF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Antibody Western blot of UBTF expression in PC-12.)

Western Blot (WB)

(Antibody Western blot of UBTF expression in HepG2.)

ELISA (EIA)

(Detection limit for recombinant GST tagged UBTF is approximately 1 ng/ml as a capture antibody.)

Related Product Information for anti-UBTF/UBF antibody

UBTF/UBF is a member of the HMG-box DNA-binding protein family. the encoded protein plays a critical role in ribosomal RNA transcription as akey component of the pre-initiation complex, mediating the recruitmentof RNA polymerase I to rDNA promoter regions. the encoded protein mayalso play important roles in chromatin remodeling and pre-rRNAprocessing, and its activity is regulated by both phosphorylation and acetylation.

NCBI and Uniprot Product Information

• NCBI GI #: 7657671
• NCBI GeneID: 7343
• NCBI Accession #: NP_055048.1
• NCBI GenBank Nucleotide #: NM_014233.3
• UniProt Accession #: P17480; A8K6R8
• Molecular Weight: 84,937 Da
• NCBI Official Full Name: nucleolar transcription factor 1 isoform a
• NCBI Official Synonym Full Names: upstream binding transcription factor
• NCBI Official Symbol: UBTF
• NCBI Official Synonym Symbols: UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• NCBI Protein Information: nucleolar transcription factor 1
• UniProt Protein Name: Nucleolar transcription factor 1
• UniProt Gene Name: UBTF
• UniProt Synonym Gene Names: UBF; UBF1; UBF-1

NCBI Description

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Uniprot Description

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.