Rabbit anti-Human, Mouse ALDH3A1 Polyclonal Antibody | anti-ALDH3A1 antibody

ALDH3A1, ID (ALDHIII, Aldehyde Dehydrogenase Family 3 Member A1, Aldehyde Dehydrogenase 3, Aldehyde Dehydrogenase, Dimeric NADP-preferring, ALDH3) (HRP)

Cat. Num. AAA6462285

• Gene Names: ALDH3A1; ALDH3; ALDHIII
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ALDH3A1; Polyclonal Antibody; ID (ALDHIII; Aldehyde Dehydrogenase Family 3 Member A1; Aldehyde Dehydrogenase 3; Aldehyde Dehydrogenase; Dimeric NADP-preferring; ALDH3) (HRP); anti-ALDH3A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH3A1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ALDH3A1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ALDH3A1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-ALDH3A1 Antibody (Center) in Y79 cell line lysates (35ug/lane). ALDH3A1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-ALDH3A1 Antibody (Center) in mouse muscle tissue lysates (35ug/lane). ALDH3A1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDH3A1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ALDH3A1 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Glatt, H., Arch. Biochem. Biophys. 477(2), 196-205 (2008). 2. Ekhart, C., Pharmacogenet. Genomics 18(6), 515-523 (2008). 3. Giebultowicz, J., Acta Pol Pharm 65(1), 81-84 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 22907049
• NCBI GeneID: 218
• NCBI Accession #: NP_000682
• NCBI GenBank Nucleotide #: NM_000691.4
• UniProt Accession #: P30838; Q9BT37; A8K828
• Molecular Weight: 50,395 Da
• NCBI Official Full Name: aldehyde dehydrogenase, dimeric NADP-preferring
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 3 family, member A1
• NCBI Official Symbol: ALDH3A1
• NCBI Official Synonym Symbols: ALDH3; ALDHIII
• NCBI Protein Information: aldehyde dehydrogenase, dimeric NADP-preferring; stomach aldehyde dehydrogenase; aldehyde dehydrogenase type III; aldehyde dehydrogenase isozyme 3
• UniProt Protein Name: Aldehyde dehydrogenase, dimeric NADP-preferring
• Protein Family: Aldehyde dehydrogenase
• UniProt Gene Name: ALDH3A1
• UniProt Synonym Gene Names: ALDH3
• UniProt Entry Name: AL3A1_HUMAN

NCBI Description

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]

Uniprot Description

ALDH3A1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. Belongs to the aldehyde dehydrogenase family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - histidine; Oxidoreductase; EC 1.2.1.5; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; extracellular space; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity

Biological Process: response to drug; response to cAMP; positive regulation of cell proliferation; response to glucocorticoid stimulus; response to hypoxia; aldehyde metabolic process; response to nutrient; aging