Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ALDH2 Polyclonal Antibody | anti-ALDH2 antibody
Goat anti-ALDH2 Antibody
Western Blot: Approx 50kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 56.4kDa according to Human NP_000681.2 amd 56.5kDa according to Mouse NP_033786.1). Recommended concentration: 0. 03-0.1ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ALDH2 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Testing Data
((1.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml.)
Testing Data
((1.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]
Uniprot Description
ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]
Protein type: Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - histidine; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Secondary Metabolites Metabolism - limonene and pinene degradation; Carbohydrate Metabolism - propanoate; EC 1.2.1.3; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - ascorbate and aldarate; Mitochondrial; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tryptophan; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 12q24.2
Cellular Component: mitochondrial matrix
Molecular Function: aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; electron carrier activity
Biological Process: alcohol metabolic process; carbohydrate metabolic process; ethanol catabolic process; ethanol oxidation
Disease: Alcohol Sensitivity, Acute
Research Articles on ALDH2
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Product Notes
The ALDH2 aldh2 (Catalog #AAA422393) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ALDH2 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 50kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 56.4kDa according to Human NP_000681.2 amd 56.5kDa according to Mouse NP_033786.1). Recommended concentration: 0. 03-0.1ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant ALDH2 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml. Researchers should empirically determine the suitability of the ALDH2 aldh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DETQFKKILG YIN. It is sometimes possible for the material contained within the vial of "ALDH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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