Rabbit anti-Human, Mouse ALDH1L1 Polyclonal Antibody | anti-ALDH1L1 antibody

ALDH1L1, CT (Aldehyde Dehydrogenase Family 1 Member L1, 10-formyltetrahydrofolate Dehydrogenase, 10-FTHFDH, FTHFD) (PE)

Cat. Num. AAA6462258

• Gene Names: ALDH1L1; FDH; FTHFD; 10-fTHF; 10-FTHFDH
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ALDH1L1; Polyclonal Antibody; CT (Aldehyde Dehydrogenase Family 1 Member L1; 10-formyltetrahydrofolate Dehydrogenase; 10-FTHFDH; FTHFD) (PE); anti-ALDH1L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH1L1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ALDH1L1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa724-754 from the C-terminal region of human ALDH1L1, conjugated to KLH.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ALDH1L1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). ALDH1L1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1L1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ALDH1L1 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Hosgood, H.D. III, Carcinogenesis 29(10), 1938-1943 (2008). 2. Lee, K.M., Hum. Genet. 122(5), 525-533 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21614513
• NCBI GeneID: 10840
• NCBI Accession #: NP_036322
• NCBI GenBank Nucleotide #: NM_012190.3
• UniProt Accession #: O75891; Q68CS1; Q8TBP8; B4DG36; E9PBX3
• Molecular Weight: 55,394 Da
• NCBI Official Full Name: cytosolic 10-formyltetrahydrofolate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member L1
• NCBI Official Symbol: ALDH1L1
• NCBI Official Synonym Symbols: FDH; FTHFD; 10-fTHF; 10-FTHFDH
• NCBI Protein Information: cytosolic 10-formyltetrahydrofolate dehydrogenase
• UniProt Protein Name: Cytosolic 10-formyltetrahydrofolate dehydrogenase
• Protein Family: Cytosolic 10-formyltetrahydrofolate dehydrogenase
• UniProt Gene Name: ALDH1L1
• UniProt Synonym Gene Names: FTHFD; 10-FTHFDH; FDH
• UniProt Entry Name: AL1L1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

ALDH1L1: 2 isoforms of the human protein are produced by alternative splicing

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; EC 1.5.1.6

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: mitochondrion

Molecular Function: methyltransferase activity; aldehyde dehydrogenase (NAD) activity; formyltetrahydrofolate dehydrogenase activity; hydroxymethyl-, formyl- and related transferase activity; catalytic activity

Biological Process: methylation; biosynthetic process; one-carbon compound metabolic process; 10-formyltetrahydrofolate catabolic process