<
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Rat Kidney Tissue.)
Rabbit anti-Rat Alanine Glyoxylate Aminotransferase 2 Polyclonal Antibody | anti-AGXT2 antibody
Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2)
Gene Names
Agxt2; AGT2
Reactivity
Rat
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Alanine Glyoxylate Aminotransferase 2; Polyclonal Antibody; Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2); AGT2; Beta-Alanine-Pyruvate Aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase; anti-AGXT2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against AGXT2. It has been selected for its ability to recognize AGXT2 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
514
Applicable Applications for anti-AGXT2 antibody
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Rat
Immunogen
Recombinant AGXT2 (Met225~Lys512) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073274
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Rat Kidney Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Rat Liver Tissue; Lane2: Rat Kidney Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant AGXT2, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant AGXT2, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,201 Da
NCBI Official Full Name
alanine--glyoxylate aminotransferase 2, mitochondrial
NCBI Official Synonym Full Names
alanine-glyoxylate aminotransferase 2
NCBI Official Symbol
Agxt2
NCBI Official Synonym Symbols
AGT2
NCBI Protein Information
alanine--glyoxylate aminotransferase 2, mitochondrial
UniProt Protein Name
Alanine--glyoxylate aminotransferase 2, mitochondrial
Protein Family
UniProt Gene Name
Agxt2
UniProt Synonym Gene Names
Agt2; AGT 2
Uniprot Description
AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.40; EC 2.6.1.44; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 2q16
Cellular Component: mitochondrial matrix; mitochondrion
Molecular Function: (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; beta-alanine-pyruvate transaminase activity; identical protein binding; pyridoxal phosphate binding
Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; L-alanine catabolic process, by transamination; positive regulation of nitric oxide biosynthetic process; pyrimidine nucleoside catabolic process
Research Articles on AGXT2
Similar Products
Product Notes
The AGXT2 agxt2 (Catalog #AAA2033904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alanine Glyoxylate Aminotransferase 2 can be used in a range of immunoassay formats including, but not limited to, WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the AGXT2 agxt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alanine Glyoxylate Aminotransferase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
