Rabbit anti-Human Akt Substrate, 160kD Polyclonal Antibody | anti-AS160 antibody

Akt Substrate, 160kD, Phosphorylated (Ser666) (AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, Rab GAP) (AP)

Cat. Num. AAA6461973

• Gene Names: IRS1; HIRS-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Akt Substrate; 160kD; Polyclonal Antibody; Phosphorylated (Ser666) (AS160; DKFZp779C0666; KIAA0603; TBC1 domain family member 4; Rab GAP) (AP); anti-AS160 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AS160 when phosphorylated on Ser666. Species sequence homology: mouse (79%), cow, horse, and dog (90%).
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-AS160 antibody: Western Blot (WB)
• Application Notes: WB: 1:250; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acids including and surrounding phosphorylated Ser666 of human AS160. Cellular Localization: Cytoplasm.
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: Untreated (lane 1) and IGF1-treated (lane 2) HEK293 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed with Anti-phospho-AS160 (Ser666) (1:250 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated AS160)

Product Categories/Family for anti-AS160 antibody

Antibodies; Protein Kinases

References

1. Chen, S. et al. (2008). Biochem. J. 409, 449-459. 2. Geraghty, K.M., et al. (2007). Biochem. J., 407:231-241.

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• UniProt Accession #: O60343; P35568
• Molecular Weight: 131,591 Da
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1; IRS-1
• UniProt Protein Name: TBC1 domain family member 4
• UniProt Gene Name: TBC1D4
• UniProt Synonym Gene Names: AS160; KIAA0603; AS160
• UniProt Entry Name: TBCD4_HUMAN

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Uniprot Description

AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue.

Protein type: GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: cytoplasmic vesicle membrane; cytoplasm

Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus

Disease: Diabetes Mellitus, Noninsulin-dependent, 5