Rabbit Akt Polyclonal Antibody | anti-Akt1 antibody

Akt, phosphorylated (Thr308) (RAC Family Serine/Threonine-protein Kinase Homolog, pkbA, akt, dagA, DDB_G0268620)

Cat. Num. AAA626750

• Gene Names: Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Akt; Polyclonal Antibody; phosphorylated (Thr308) (RAC Family Serine/Threonine-protein Kinase Homolog; pkbA; akt; dagA; DDB_G0268620); Anti -Akt; anti-Akt1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogeneous levels of human Akt only when phosphorylated at Thr308. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Akt1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:200; Western Blot: 1:500-1:1000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human Akt around the phosphorylation site of threonine 308 (M-K-pT-F-C).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Akt1 antibody

AKT protein family, which members are also called protein kinases B (PKB) plays an important role in mammalian cellular signaling. In humans, there are three genes in the Akt family: Akt1, Akt2 and Akt3. These genes code for enzymes that are members of the serine/threonine-specific protein kinase family. Akt1 is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt1 is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Akt (now also called Akt1) was originally identified as the oncogene in the transforming retrovirus, AKT8 (1). AKT8 was isolated by Stephen Staal in the laboratory of Wallace P. Rowe; he ubsequently cloned v-akt and human AKT1 and AKT2 while on staff at the Johns Hopkins Oncology Center (2). Akt2 is an important signaling molecule in the Insulin signaling pathway, it is required to induce glucose transport. Franke et al. (1995) show that AKT1 and AKT2 are activated by PDGF. The activation was rapid and specific, and it was abrogated by mutations in the Akt Pleckstrin homology (PH) domain. They identify that Akt is a novel target of PI 3-kinase and suggest that the Akt PH domain may be a mediator of PI 3-kinase signaling.

Product Categories/Family for anti-Akt1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 24647360
• NCBI GeneID: 41957
• NCBI Accession #: NP_732115.1
• NCBI GenBank Nucleotide #: NM_169707.1
• UniProt Accession #: Q8INB9; Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7
• Molecular Weight: 68,485 Da
• NCBI Official Full Name: Akt1, isoform B
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
• NCBI Protein Information: CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
• UniProt Protein Name: RAC serine/threonine-protein kinase
• Protein Family: AKT-interacting protein
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: DAkt; DRAC-PK; Dakt1; PKB
• UniProt Entry Name: AKT1_DROME

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Protein kinase, AGC; Oncoprotein; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: oogenesis; circadian rhythm; positive regulation of cell size; regulation of dendrite development; chitin-based embryonic cuticle biosynthetic process; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; epithelial cell migration, open tracheal system; positive regulation of cell growth; positive regulation of organ growth; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; lipid metabolic process; response to oxidative stress; regulation of cell size; negative regulation of apoptosis