Rabbit AKT Polyclonal Antibody | anti-AKT antibody
AKT (C20) Antibody
Reactivity
Mouse, rat, human
Applications
Western Blot, Immunohistochemistry
Synonyms
AKT; Polyclonal Antibody; AKT (C20) Antibody; anti-AKT antibody
Host
Rabbit
Reactivity
Mouse, rat, human
Clonality
Polyclonal
Specificity
Mouse, rat, and human AKT
Form/Format
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
Concentration
0.200 mg/ml (varies by lot)
Sequence Length
286
Applicable Applications for anti-AKT antibody
Western Blot, Immunohistochemistry (IHC)
Origin
Akt1 (C20) is provided as an affinity purified rabbit polyclonal antibody, raised against a synthetic peptide mapping to the carboxy terminal domain of human Akt1.
Immunogen
Synthetic peptide mapping to the carboxy terminal domain of human AKT1. Differs from corresponding mouse sequence by one residue.
Preparation and Storage
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
Related Product Information for anti-AKT antibody
Akt1, also known as Protein kinase B, is a serine/threonine kinase that is at the center of many signal transduction pathways. Akt1 regulates glucose metabolism, transcription, apoptosis, cell proliferation, angiogenesis, and cell motility (1). The activation of Akt1 occurs downstream of PI-3K, which upon activation by an extracellular molecule, generates PIP (3) (2). PIP (3) is a lipid second messenger that promotes the translocation of Akt1 to the plasma membrane via PH (pleckstrin-homology) domain binding (2-4). At the plasma membrane Akt1 is phosphorylated and activated by PDK-1 at its catalytic site on residue Thr308 (1). Residue Ser473 is also phosphorylated at this time but the kinase has not yet been identified (1). In this activated form Akt1 phosphorylates proteins that contain the motif, R-x-R-x-x-S/T-F/L, at the plasma membrane, in the cytosol, and in the nucleus (1). In addition to its kinase activity, Akt1 forms complexes with other proteins that are not substrates. These other proteins act as modulators of Akt1 activity (1).
References
1. Brazil DP, Park J, Hemmings BA. 2002. PKB binding proteins. Getting in on the Akt. Cell 111 (3):293-303.2. Nicholson KM, Anderson NG. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14(5): 381-395.3. Marte BM, Downward J. 1997. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci 22(9): 355-358.4. Coffer PJ, Jin J, Woodgett JR. 1998. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 335(Pt1): 1-13.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,995 Da
NCBI Official Full Name
Akt, partial
UniProt Protein Name
Akt
Protein Family
UniProt Gene Name
Akt
UniProt Entry Name
Q66XT6_ANOST
Similar Products
Product Notes
The AKT akt (Catalog #AAA440053) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKT (C20) Antibody reacts with Mouse, rat, human and may cross-react with other species as described in the data sheet. AAA Biotech's AKT can be used in a range of immunoassay formats including, but not limited to, Western Blot, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the AKT akt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
