Rabbit Akirin2 Polyclonal Antibody | anti-AKIRIN2 antibody

Akirin2 Antibody

Cat. Num. AAA151488

• Gene Names: AKIRIN2; FBI1; C6orf166; dJ486L4.2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Akirin2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Akirin2; Polyclonal Antibody; Akirin2 Antibody; FBI1; C6orf166; dJ486L4.2; Akirin-2; akirin 2; anti-AKIRIN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Akirin2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 203

• Applicable Applications for anti-AKIRIN2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Akirin2 antibody can be used for detection of Akirin2 by Western blot at 0.5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Akirin2 antibody was raised against a 15 amino acid synthetic peptide near the center of the human Akirin2.
• Buffer: Akirin2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Akirin2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Akirin2 in Human Brain tissue lysate with Akirin2 antibody at 0.5 μg/mL in (A) the absence and (B) the presence ofblocking peptide.)

Related Product Information for anti-AKIRIN2 antibody

Akirin2 Antibody: The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-κB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-κB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-α and IL-1β signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.

NCBI and Uniprot Product Information

• NCBI GI #: 119568963
• NCBI GeneID: 55122
• UniProt Accession #: Q53H80; Q9BQB1
• Molecular Weight: 22,496 Da
• NCBI Official Full Name: chromosome 6 open reading frame 166
• NCBI Official Synonym Full Names: akirin 2
• NCBI Official Symbol: AKIRIN2
• NCBI Official Synonym Symbols: FBI1; C6orf166; dJ486L4.2
• NCBI Protein Information: akirin-2; fourteen-three-three beta interactant 1
• UniProt Protein Name: Akirin-2
• Protein Family: Akirin
• UniProt Gene Name: AKIRIN2
• UniProt Synonym Gene Names: C6orf166
• UniProt Entry Name: AKIR2_HUMAN

Uniprot Description

AKIRIN2: Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6. Forms a complex with YWHAB that acts to repress transcription of DUSP1. Belongs to the akirin family.

Protein type: Cell cycle regulation; Transcription regulation

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: enzyme binding

Biological Process: embryonic development; transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of interleukin-6 production; innate immune response; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter