Typical Testing Data/Standard Curve
Rabbit Akirin-2 (AKIRIN2) ELISA Kit | AKIRIN2 elisa kit
Rabbit Akirin-2 (AKIRIN2) ELISA Kit
Gene Names
AKIRIN2; FBI1; C6orf166; dJ486L4.2
Reactivity
Rabbit
Synonyms
Akirin-2 (AKIRIN2); Rabbit Akirin-2 (AKIRIN2) ELISA Kit; AKIRIN2 elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for AKIRIN2 elisa kit
Intended Uses: This AKIRIN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit AKIRIN2. This ELISA kit for research use only!
Principle of the Assay||AKIRIN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AKIRIN2 antibody and an AKIRIN2-HRP conjugate. The assay sample and buffer are incubated together with AKIRIN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKIRIN2 concentration since AKIRIN2 from samples and AKIRIN2-HRP conjugate compete for the anti-AKIRIN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKIRIN2 from the sample, fewer sites are left to bind AKIRIN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKIRIN2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||AKIRIN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AKIRIN2 antibody and an AKIRIN2-HRP conjugate. The assay sample and buffer are incubated together with AKIRIN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKIRIN2 concentration since AKIRIN2 from samples and AKIRIN2-HRP conjugate compete for the anti-AKIRIN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKIRIN2 from the sample, fewer sites are left to bind AKIRIN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKIRIN2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AKIRIN2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,496 Da
NCBI Official Full Name
akirin-2
NCBI Official Synonym Full Names
akirin 2
NCBI Official Symbol
AKIRIN2
NCBI Official Synonym Symbols
FBI1; C6orf166; dJ486L4.2
NCBI Protein Information
akirin-2; fourteen-three-three beta interactant 1
UniProt Protein Name
Akirin-2
Protein Family
UniProt Gene Name
AKIRIN2
UniProt Synonym Gene Names
C6orf166
UniProt Entry Name
AKIR2_HUMAN
Uniprot Description
AKIRIN2: Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6. Forms a complex with YWHAB that acts to repress transcription of DUSP1. Belongs to the akirin family.
Protein type: Cell cycle regulation; Transcription regulation
Chromosomal Location of Human Ortholog: 6q15
Cellular Component: transcriptional repressor complex; nucleus
Molecular Function: enzyme binding
Biological Process: embryonic development; transcription, DNA-dependent; positive regulation of cell proliferation; innate immune response; positive regulation of interleukin-6 production; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter
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Product Notes
The Rabbit AKIRIN2 akirin2 (Catalog #AAA7231711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231711 ELISA Kit recognizes Rabbit AKIRIN2. It is sometimes possible for the material contained within the vial of "Akirin-2 (AKIRIN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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