Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using AKAP8L Antibody. The lane on the left was treated with blocking peptide.)
Rabbit AKAP8L Polyclonal Antibody | anti-AKAP8L antibody
AKAP8L Antibody
Reactivity
HumanPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
AKAP8L; Polyclonal Antibody; AKAP8L Antibody; A kinase (PRKA) anchor protein 8 like; A kinase anchor protein 8 like; AKAP 8L; AKAP8 like protein; DKFZp434L0650; HA95; HAP95; Helicase A binding protein 95; Homologous to AKAP95 protein; NAKAP; NAKAP95; Neighbor of A kinase anchoring protein 95; Neighbor of AKAP95; anti-AKAP8L antibody
Host
Rabbit
Reactivity
Human
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
AKAP8L Antibody detects endogenous levels of AKAP8L.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
646
Applicable Applications for anti-AKAP8L antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human AKAP8L.
Tissue Specificity
Ubiquitously expressed. Expressed in the brain cortex (at protein level).
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using AKAP8L Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using AKAP8L Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-AKAP8L antibody
Description: Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).
Post Translational Modifications: Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.
Subunit Structure: Interacts (via N-terminus) with DHX9 (via RGG region) (PubMed:10748171, PubMed:11402034). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B (PubMed:11034899, PubMed:12538639 PubMed:16391387, PubMed:18313049). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with EBV EBNA-LP (PubMed:11160753). Interacts with HIV-1 reverse transcriptase/ribonuclease H (PubMed:25034436).
Similarity: Belongs to the AKAP95 family.
Post Translational Modifications: Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.
Subunit Structure: Interacts (via N-terminus) with DHX9 (via RGG region) (PubMed:10748171, PubMed:11402034). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B (PubMed:11034899, PubMed:12538639 PubMed:16391387, PubMed:18313049). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with EBV EBNA-LP (PubMed:11160753). Interacts with HIV-1 reverse transcriptase/ribonuclease H (PubMed:25034436).
Similarity: Belongs to the AKAP95 family.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
Observed: 72 kDa
Predicted: 72 kDa
Predicted: 72 kDa
NCBI Official Full Name
A-kinase anchor protein 8-like
UniProt Protein Name
A-kinase anchor protein 8-like
Protein Family
UniProt Gene Name
AKAP8L
UniProt Synonym Gene Names
NAKAP; NAKAP95; AKAP8-like protein; HAP95; HA95; Neighbor of AKAP95
UniProt Entry Name
AKP8L_HUMAN
Uniprot Description
AKAP8L: Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta. Belongs to the AKAP95 family.
Protein type: RNA-binding; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus
Molecular Function: protein binding; DEAD/H-box RNA helicase binding; DNA binding; metal ion binding
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Product Notes
The AKAP8L akap8l (Catalog #AAA9611569) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP8L Antibody reacts with Human Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP8L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the AKAP8L akap8l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP8L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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