Western Blot (WB)
(Western Blot (WB) analysis of AKAP3 polyclonal antibody at 1:500Line1:Hela whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Rabbit AKAP3 Polyclonal Antibody | anti-AKAP3 antibody
AKAP3 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
AKAP3; Polyclonal Antibody; AKAP3 Polyclonal Antibody; A-kinase anchor protein 3; AKAP-3; A-kinase anchor protein 110 kDa; AKAP 110; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; FSP95; Fibrousheathin I; Fibrousheathin-1; Protein kinase A-anchoring protein 3; PRKA3; Sperm oocyte-binding protein; AKAP110; SOB1; anti-AKAP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AKAP3 polyclonal antibody detects endogenous levels of AKAP3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
853
Applicable Applications for anti-AKAP3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human AKAP3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of AKAP3 polyclonal antibody at 1:500Line1:Hela whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of AKAP3 polyclonal antibody at 1:500Line1:Hela whole cell lysateLine2:H9C2 whole cell lysateLine3:Raw264. 7 whole cell lysate)
Related Product Information for anti-AKAP3 antibody
The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP). AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA. AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 95kDa
NCBI Official Full Name
A-kinase anchor protein 3
UniProt Protein Name
A-kinase anchor protein 3
Protein Family
UniProt Gene Name
AKAP3
UniProt Synonym Gene Names
AKAP110; SOB1; AKAP-3; AKAP 110; CT82; FSP95; PRKA3
UniProt Entry Name
AKAP3_HUMAN
Uniprot Description
AKAP3: May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction. Belongs to the AKAP110 family.
Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 12p13.3
Cellular Component: cytoplasm; acrosome; nucleus
Molecular Function: protein binding; protein kinase A binding
Biological Process: protein localization; single fertilization; acrosome reaction; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility
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Product Notes
The AKAP3 akap3 (Catalog #AAA3007176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the AKAP3 akap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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