Goat AKAP12 Polyclonal Antibody | anti-Akap12 antibody
Goat anti-AKAP12 (mouse) Antibody
Gene Names
Akap12; Srcs5; SSeCKS; Tsga12; AI317366
Reactivity
Expected from sequence similarity: Mouse, Rat
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AKAP12; Polyclonal Antibody; Goat anti-AKAP12 (mouse) Antibody; Akap12; A kinase (PRKA) anchor protein (gravin) 12; AI317366; SSeCKS; Srcs5; Tsga12; A-kinase anchor protein 12; AKAP-12; gercelin; germ cell lineage protein gercelin; src-suppressed C kinase substrate; testis specific gene A12; v-src suppressed transcri; Akap12 antibody; A kinase (PRKA) anchor protein (gravin) 12 antibody; AI317366 antibody; SSeCKS antibody; Srcs5 antibody; Tsga12 antibody; A-kinase anchor protein 12 antibody; AKAP-12 antibody; gercelin antibody; germ cell lineage protein gercelin antibody; src-suppressed C kinase substrate antibody; testis specific gene A12 antibody; v-src suppressed transcript 5 antibody; AKAP12 (mouse); anti-Akap12 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SPDTNGPKLTEEGD
Sequence Length
1684
Applicable Applications for anti-Akap12 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2ug/ml).
Western Blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2ug/ml).
Immunogen
Peptide with sequence C-SPDTNGPKLTEEGD, from the internal region of the protein sequence according to NP_112462.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform 1 (NP_005091.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
169,743 Da
NCBI Official Full Name
A-kinase anchor protein 12
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein (gravin) 12
NCBI Official Symbol
Akap12
NCBI Official Synonym Symbols
Srcs5; SSeCKS; Tsga12; AI317366
NCBI Protein Information
A-kinase anchor protein 12
UniProt Protein Name
A-kinase anchor protein 12
Protein Family
UniProt Gene Name
Akap12
UniProt Synonym Gene Names
Gag12; Ssecks; AKAP-12; SSeCKS
UniProt Entry Name
AKA12_MOUSE
Uniprot Description
AKAP12: a scaffold protein that contains 3 conserved PKA binding motifs. Suppresses podosome formation, inhibits RhoA and Cdc42 activity, and inhibits v-Src-induced oncogenic growth in NIH3T3. May inhibit Src-induced oncogenesis by reestablishing actin-based non-invasive cytoskeletal architecture.
Protein type: Adaptor/scaffold
Cellular Component: cytoplasm; cytoskeleton; focal adhesion; membrane; plasma membrane
Molecular Function: adenylate cyclase binding; protein kinase A binding; receptor signaling complex scaffold activity
Biological Process: positive regulation of cAMP biosynthetic process; protein targeting; signal transduction
Research Articles on Akap12
Similar Products
Product Notes
The Akap12 akap12 (Catalog #AAA422787) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AKAP12 (mouse) Antibody reacts with Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP12 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2ug/ml). Researchers should empirically determine the suitability of the Akap12 akap12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SPDTNGPKLT EEGD. It is sometimes possible for the material contained within the vial of "AKAP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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