Rabbit AKAP10 Polyclonal Antibody | anti-AKAP10 antibody

AKAP10 Antibody

Cat. Num. AAA9600265

• Gene Names: AKAP10; PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: AKAP10; Polyclonal Antibody; AKAP10 Antibody; 1500031L16Rik; A kinase (PRKA) anchor protein 10; A kinase anchor protein 10; A kinase anchor protein 10 mitochondrial; A-kinase anchor protein 10; AKA10_HUMAN; akap 10; AKAP-10; B130049N18Rik; D AKAP 2; D-AKAP-2; dakap 2; dakap2; Dual specific A kinase anchoring protein 2; Dual specificity A kinase-anchoring protein 2; mgc9410; MGC9414|; Mitochondrial A kinase PPKA anchor protein 10; mitochondrial; prka 10; PRKA10; Protein A kinase anchoring protein 10; Protein A kinase anchoring protein 10 mitochondrial precursor; Protein kinase A anchoring protein 10; Protein kinase A-anchoring protein 10; anti-AKAP10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AKAP10 antibody detects endogenous levels of total AKAP10
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 662

• Applicable Applications for anti-AKAP10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:3000
• Immunogen: A synthesized peptide derived from human AKAP10
• Subcellular Location: Mitochondrion. Membrane. Cytoplasm. Predominantly mitochondrial but also membrane associated and cytoplasmic.
• Predicted Cross Reactivity: Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Bovine (91%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on mouse muscle lysate using AKAP10 Antibody)

Western Blot (WB)

(Western blot analysis on mouse brain cell lysate using AKAP10 Antibody.The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-AKAP10 antibody

Description: AKAP10 Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA.
Function: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase (By similarity).
Similarity: RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

NCBI and Uniprot Product Information

• NCBI GI #: 21493033
• NCBI GeneID: 11216
• NCBI Accession #: NP_009133.2
• NCBI GenBank Nucleotide #: NM_007202.3
• UniProt Accession #: O43572; Q96AJ7; B2R650
• Molecular Weight: Observed: 73 kDa; Predicted: 74 kDa
• NCBI Official Full Name: A-kinase anchor protein 10, mitochondrial isoform 1
• NCBI Official Synonym Full Names: A-kinase anchoring protein 10
• NCBI Official Symbol: AKAP10
• NCBI Official Synonym Symbols: PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
• NCBI Protein Information: A-kinase anchor protein 10, mitochondrial
• UniProt Protein Name: A-kinase anchor protein 10, mitochondrial
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP10
• UniProt Synonym Gene Names: AKAP-10; D-AKAP-2; PRKA10

NCBI Description

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012]

Uniprot Description

Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase ().