Rabbit AK6 Polyclonal Antibody | anti-AK6 antibody

AK6 (G35) Polyclonal Antibody

Cat. Num. AAA3002230

• Gene Names: AK6; CIP; CINAP; AD-004; hCINAP; CGI-137
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AK6; Polyclonal Antibody; AK6 (G35) Polyclonal Antibody; Adenylate kinase isoenzyme 6; Adrenal gland protein AD-004; Coilin-interacting nuclear ATPase protein; hCINAP; Dual activity adenylate kinase/ATPase; AK/ATPase; CINAP; AD-004; CGI-137; anti-AK6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AK6 (G35) polyclonal antibody detects endogenous levels of AK6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 169

• Applicable Applications for anti-AK6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to the N-terminual of Human AK6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AK6 (G35) polyclonal antibody at 1:1000 dilutionLane1:A549 whole cell lysate (40ug) Lane2:The Lung tissue lysate of Rat (30ug) Lane3:The Lung tissue lysate of Mouse (30ug))

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using AK6  (G35)  polyclonal antibody (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using ATP5H  (D146) # MBS3002230.“up-arrow” (supernatant) ;“down-arrow (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of AK6 (G35) pAb at 1:500 dilutionLane1:H1792 whole cell lysate (40ug) Lane2:COS-7 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug))

Related Product Information for anti-AK6 antibody

TAF9(AK6) functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).

NCBI and Uniprot Product Information

• NCBI GI #: 62865612
• NCBI GeneID: 102157402
• NCBI Accession #: NP_001015891.1
• NCBI GenBank Nucleotide #: NP_001015891.1
• UniProt Accession #: Q9Y3D8
• Molecular Weight: ~ 20kDa
• NCBI Official Full Name: adenylate kinase isoenzyme 6 isoform c
• NCBI Official Synonym Full Names: adenylate kinase 6
• NCBI Official Symbol: AK6
• NCBI Official Synonym Symbols: CIP; CINAP; AD-004; hCINAP; CGI-137
• NCBI Protein Information: adenylate kinase isoenzyme 6
• UniProt Protein Name: Adenylate kinase isoenzyme 6
• Protein Family: Adenylate kinase
• UniProt Gene Name: TAF9
• UniProt Synonym Gene Names: AK6; CINAP; AK6; hCINAP; AK/ATPase
• UniProt Entry Name: KAD6_HUMAN

NCBI Description

This gene encodes a protein that belongs to the adenylate kinase family of enzymes. The protein has a nuclear localization and contains Walker A (P-loop) and Walker B motifs and a metal-coordinating residue. The protein may be involved in regulation of Cajal body formation. In human, AK6 and TAF9 (GeneID: 6880) are two distinct genes that share 5' exons. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

TAFII31 iso2: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.

Protein type: Kinase, other; Transcription initiation complex; Acetyltransferase; DNA-binding; EC 2.7.4.3

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; transcription activator binding; p53 binding; protein heterodimerization activity; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; protein stabilization; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis