Goat AIF1 / IBA1 Polyclonal Antibody | anti-AIF1 antibody

Goat Polyclonal to Human AIF1 / IBA1

Cat. Num. AAA242027

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: AIF1 / IBA1; Polyclonal Antibody; Goat Polyclonal to Human AIF1 / IBA1; Anti-AIF1 / IBA1 Antibody (C-Terminus) IHC-plus; AIF1; AIF-1; Em:AF129756.17; G1; IRT-1; Protein G1; IBA1; IRT1; Human AIF1; anti-AIF1 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human AIF1 / IBA1. This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 132

• Applicable Applications for anti-AIF1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (2.5 ug/ml), WB (1:16000, 1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-YEEKAREKEKP from the C-terminus of human AIF1 / IBA1 (NP_116573.1; NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (91%).
• Immunogen Type: Synthetic peptide
• Immunogen: AIF1 / IBA1 antibody was raised against synthetic peptide C-YEEKAREKEKP from the C-terminus of human AIF1 / IBA1 (NP_116573.1; NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (91%); Goat, Platypus, Slime mold, Spikemoss, Chaetomium (82%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-AIF1 / IBA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of Human Lymph Node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-AIF1 antibody

Allograft inflammatory Factor 1 (AIF1) is a calcium binding peptide produced by activated monocytes and microglial cells. AIF1 expression is associated with chronic inflammatory processes and is also transiently expressed in response to vascular trauma.

NCBI and Uniprot Product Information

• NCBI GI #: 4758612
• NCBI GeneID: 199
• NCBI Accession #: NP_004838.1
• NCBI GenBank Nucleotide #: NM_004847.3
• UniProt Accession #: P55008; O43904; Q9UIV4; Q9UKS9; A8K406
• Molecular Weight: 14,617 Da
• NCBI Official Full Name: allograft inflammatory factor 1 isoform 2
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; perikaryon; actin filament; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; inflammatory response; response to electrical stimulus; negative regulation of apoptosis