Rabbit AIF1 Polyclonal Antibody | anti-AIF1 antibody

Anti-AIF1 Antibody

Cat. Num. AAA415525

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Human. Predicted: Mouse, Rat.
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: AIF1; Polyclonal Antibody; Anti-AIF1 Antibody; anti-AIF1 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat.
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 147

• Applicable Applications for anti-AIF1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:500-1:1,000; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal AIF1 (1) antibody was raised against a peptide from C terminal residues of human AIF1 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-AIF1 antibody

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

References

Yamamoto,A., Ashihara,E., Nakagawa,Y. et al. Allograft inflammatory factor-1 is overexpressed and induces fibroblast chemotaxis in the skin of sclerodermatous GVHD in a murine model. Immunol. Lett. 135 (1-2), 144-150 (2011).

NCBI and Uniprot Product Information

• NCBI GI #: 14574568
• NCBI GeneID: 199
• NCBI Accession #: NP_001614.3
• NCBI GenBank Nucleotide #: NM_001623.3
• Molecular Weight: 14,617 Da
• NCBI Official Full Name: allograft inflammatory factor 1 isoform 3
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; allograft inflammatory factor-1 splice variant Hara-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; protein G1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; cytosol; nucleus; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; phagocytosis, engulfment; inflammatory response; response to electrical stimulus; negative regulation of apoptosis