Rabbit anti-Human, Mouse AIF1 Polyclonal Antibody | anti-AIF1 antibody

AIF1, NT (AIF1, G1, IBA1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1) (MaxLight 550)

Cat. Num. AAA6272395

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AIF1; Polyclonal Antibody; NT (AIF1; G1; IBA1; Allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1) (MaxLight 550); Protein G1; anti-AIF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes huma AIF1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-AIF1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 6-36 in the N-terminal region of human AIF1, UniProt Accession: P55008.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot using MBS645437 (1:2000) Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 17kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot using MBS645437 (1:2000) Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: human spleen lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 17kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS645437 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS645437 for immunohistochemistry.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS645437 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS645437 for immunohistochemistry.)

Product Categories/Family for anti-AIF1 antibody

Antibodies; Adapter Proteins

References

1. Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010). 2. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). 3. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 4. Jia, J., et al. Pediatr. Res. 67(1):29-34(2010). 5. Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14574568
• NCBI GeneID: 199
• NCBI Accession #: NP_001614.3
• NCBI GenBank Nucleotide #: NM_001623.3
• UniProt Accession #: P55008; O43904; Q9UIV4; Q9UKS9; A8K406
• Molecular Weight: 14,617 Da
• NCBI Official Full Name: allograft inflammatory factor 1 isoform 3
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; allograft inflammatory factor-1 splice variant Hara-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; protein G1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; response to electrical stimulus; inflammatory response; negative regulation of apoptosis