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Western Blot (WB) (Western blot analysis of lysate from Hela cell line, using Phospho-human-AHCTF1 (S1232). ctrl. MBS9203401 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Rabbit anti-Human AHCTF1 (S1232) Polyclonal Antibody | anti-AHCTF1 antibody

Human-AHCTF1 (S1232) Antibody

Gene Names
AHCTF1; ELYS; MST108; TMBS62; MSTP108
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AHCTF1 (S1232); Polyclonal Antibody; Human-AHCTF1 (S1232) Antibody; Protein ELYS; Embryonic large molecule derived from yolk sac; Protein MEL-28; Putative AT-hook-containing transcription factor 1; AHCTF1; ELYS; TMBS62; anti-AHCTF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1210-1260 amino acids from human.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
2,275
Applicable Applications for anti-AHCTF1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
Cytoplasm. Nucleus matrix. Chromosome, centromere, kinetochore. Nucleus, nucleoplasm. Nucleus, nuclear pore complex. Note=Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107- 160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from Hela cell line, using Phospho-human-AHCTF1 (S1232). ctrl. MBS9203401 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB) (Western blot analysis of lysate from Hela cell line, using Phospho-human-AHCTF1 (S1232). ctrl. MBS9203401 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-AHCTF1 antibody
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis.
Product Categories/Family for anti-AHCTF1 antibody
References
Kimura N.,et al.Genes Cells 7:435-446(2002).
Lightfoot J.,et al.Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Liu B.,et al.Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
252498
NCBI Official Full Name
protein ELYS
NCBI Official Synonym Full Names
AT hook containing transcription factor 1
NCBI Official Symbol
AHCTF1
NCBI Official Synonym Symbols
ELYS; MST108; TMBS62; MSTP108
NCBI Protein Information
protein ELYS
UniProt Protein Name
Protein ELYS
UniProt Gene Name
AHCTF1
UniProt Synonym Gene Names
ELYS; TMBS62
UniProt Entry Name
ELYS_HUMAN

Uniprot Description

ELYS: Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. Belongs to the ELYS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; nuclear membrane; nuclear matrix; nuclear pore; nucleus; cytosol; chromatin

Molecular Function: DNA binding; transcription factor activity

Biological Process: nuclear pore complex assembly; protein transport; mRNA transport; regulation of transcription, DNA-dependent; cytokinesis; mitotic cell cycle

Research Articles on AHCTF1

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Product Notes

The AHCTF1 ahctf1 (Catalog #AAA9203401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Human-AHCTF1 (S1232) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AHCTF1 (S1232) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the AHCTF1 ahctf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHCTF1 (S1232), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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