Rabbit Aha2 Polyclonal Antibody | anti-HA2 antibody

Aha2 (ATPase Hydrogen-exporting ATPase 2)

Cat. Num. AAA6007789

• Gene Names: HA2; AHA2; F9N11.40; F9N11_40; H(+)-ATPase 2; HA2; P-TYPE H(+)-ATPASE ISOFORM 2; PLASMA MEMBRANE PROTON ATPASE 2; PMA2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Aha2; Polyclonal Antibody; Aha2 (ATPase Hydrogen-exporting ATPase 2); Anti -Aha2 (ATPase Hydrogen-exporting ATPase 2); anti-HA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes Aha2. Species Crossreactivity: Human, mouse and rat. Cross-reacts with Aha1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-HA2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length Aha2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HA2 antibody

Aha2 shares 45% sequence homology with Aha1, however, little is known about the functional protein. Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Product Categories/Family for anti-HA2 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 114335
• NCBI GeneID: 829142
• UniProt Accession #: P19456
• Molecular Weight: 104,401 Da
• NCBI Official Full Name: ATPase 2, plasma membrane-type
• NCBI Official Symbol: HA2
• NCBI Official Synonym Symbols: AHA2; F9N11.40; F9N11_40; H(+)-ATPase 2; HA2; P-TYPE H(+)-ATPASE ISOFORM 2; PLASMA MEMBRANE PROTON ATPASE 2; PMA2
• NCBI Protein Information: H(+)-ATPase 2
• UniProt Protein Name: ATPase 2, plasma membrane-type
• UniProt Gene Name: AHA2
• UniProt Entry Name: PMA2_ARATH

NCBI Description

belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport. has two autoinhibitory regions within the C-terminal dom

Uniprot Description

Function: The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity: ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subunit structure: Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-947. Binding to 14-3-3 proteins activates the H+-ATPase.

Subcellular location: Cell membrane; Multi-pass membrane protein

Probable.

Tissue specificity: Higher levels in roots than in shoots.

Post-translational modification: Phosphorylation of Thr-947 induces the binding to 14-3-3 proteins, but phosphorylation of Ser-931 interfers with this binding no matter whether Thr-947 is phosphrylated or not. Phosphorylation level varies significantly during early response to general elicitors.

Miscellaneous: The catalytic mechanism involves at least four different enzyme conformational states named E1, E1P, E2P, and E2, with the E1P-E2P transition accompanying the transfer of ion across the membrane. E1P and E2P are phosphorylated intermediates.

Sequence similarities: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]