Rabbit Aha1 Polyclonal Antibody | anti-Ahsa1 antibody

Aha1 Antibody: HRP

Cat. Num. AAA806525

• Gene Names: Ahsa1; p38; BC023857
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Synonyms: Aha1; Polyclonal Antibody; Aha1 Antibody: HRP; SHSA1; HspC322; p38; anti-Ahsa1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein A Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 338

• Applicable Applications for anti-Ahsa1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: 0.5-1:1000 (WB), 1:10,000 using Antibody Amplifier (IHC)
• Conjugate: HRP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of multiple Cell line lysates showing detection of AHA1 protein using Rabbit Anti-AHA1 Polyclonal Antibody (SPC-183). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AHA1 Polyclonal Antibody (SPC-183) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Related Product Information for anti-Ahsa1 antibody

Background Info: Detects ~ 38kDa

Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Product Categories/Family for anti-Ahsa1 antibody

Chaperones; Heat Shock

References

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

NCBI and Uniprot Product Information

• NCBI GI #: 22122515
• NCBI GeneID: 217737
• NCBI Accession #: NP_666148.1
• NCBI GenBank Nucleotide #: NM_146036.1
• UniProt Accession #: Q8BK64; Q8R3E6
• Molecular Weight: 38,117 Da
• NCBI Official Full Name: activator of 90 kDa heat shock protein ATPase homolog 1
• NCBI Official Synonym Full Names: AHA1, activator of heat shock protein ATPase 1
• NCBI Official Symbol: Ahsa1
• NCBI Official Synonym Symbols: p38; BC023857
• NCBI Protein Information: activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1
• UniProt Protein Name: Activator of 90 kDa heat shock protein ATPase homolog 1
• Protein Family: Activator of 90 kDa heat shock protein ATPase
• UniProt Gene Name: Ahsa1
• UniProt Synonym Gene Names: AHA1
• UniProt Entry Name: AHSA1_MOUSE

Uniprot Description

AHSA1: Cochaperone that stimulates HSP90 ATPase activity. May affect a step in the endoplasmic reticulum to Golgi trafficking. Belongs to the AHA1 family.

Protein type: Chaperone

Cellular Component: endoplasmic reticulum; cytoplasm

Molecular Function: ATPase activator activity; chaperone binding

Biological Process: positive regulation of ATPase activity; response to stress