Rat Aha1 Monoclonal Antibody | anti-Ahsa1 antibody

Aha1 Antibody: RPE

Cat. Num. AAA803854

• Gene Names: Ahsa1; p38; BC023857
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Synonyms: Aha1; Monoclonal Antibody; Aha1 Antibody: RPE; Aha 1; Ahsa1; p38; HspC322; C14orf3; Activator of Hsp90 ATPase; Activator of 90 kDa heat shock protein ATPase homolog 1; anti-Ahsa1 antibody

• Host: Rat
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone Number: 25F2.D9
• Form/Format: Protein G Purified
• Concentration: 1.2mg/mL (varies by lot)
• Sequence Length: 338

• Applicable Applications for anti-Ahsa1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Conjugate: RPE
• Storage Buffer: 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of Aha1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Related Product Information for anti-Ahsa1 antibody

Background Info: Detects ~38kDa. Can run up to 45kDa on SDS Page

Scientific Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Product Categories/Family for anti-Ahsa1 antibody

Chaperones; Heat Shock

References

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

NCBI and Uniprot Product Information

• NCBI GI #: 22122515
• NCBI GeneID: 217737
• NCBI Accession #: NP_666148.1
• NCBI GenBank Nucleotide #: NM_146036.1
• UniProt Accession #: Q8BK64; Q8R3E6
• Molecular Weight: 38,117 Da
• NCBI Official Full Name: activator of 90 kDa heat shock protein ATPase homolog 1
• NCBI Official Synonym Full Names: AHA1, activator of heat shock protein ATPase 1
• NCBI Official Symbol: Ahsa1
• NCBI Official Synonym Symbols: p38; BC023857
• NCBI Protein Information: activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1
• UniProt Protein Name: Activator of 90 kDa heat shock protein ATPase homolog 1
• Protein Family: Activator of 90 kDa heat shock protein ATPase
• UniProt Gene Name: Ahsa1
• UniProt Synonym Gene Names: AHA1
• UniProt Entry Name: AHSA1_MOUSE

Uniprot Description

AHSA1: Cochaperone that stimulates HSP90 ATPase activity. May affect a step in the endoplasmic reticulum to Golgi trafficking. Belongs to the AHA1 family.

Protein type: Chaperone

Cellular Component: endoplasmic reticulum; cytoplasm

Molecular Function: ATPase activator activity; chaperone binding

Biological Process: positive regulation of ATPase activity; response to stress